Fujita K, Imoto S, Phuchareon J, Tokuhisa T
Department of Immunology, ICMR, Kobe University School of Medicine, Japan.
Immunobiology. 1994 Feb;190(1-2):13-22. doi: 10.1016/s0171-2985(11)80280-9.
We analyzed the expression of c-fos and jun family gene in murine pre-B cells developed in the interleukin-7-(IL-7) dependent bone marrow cell culture. The c-fos gene was expressed in the pre-B cells. The c-fos RNA became undetectable after IgM+ B cells were developed in the culture. The c-fos expression in the pre-B cells was IL-7-dependent. However, the c-fos RNA was not induced when the pre-B cells cultured without IL-7 for 6 h were restimulated with IL-7. The expression of c-jun RNA was slightly induced in the restimulated pre-B cells. The junB and junD RNA were the steady state level in the cells. These gene products formed AP-1 molecule in the pre-B cells. These results suggest that the AP-1 plays a role in the IL-7-dependent pre-B cell development.
我们分析了在白细胞介素-7（IL-7）依赖的骨髓细胞培养物中发育的小鼠前B细胞中c-fos和jun家族基因的表达。c-fos基因在前B细胞中表达。在培养物中发育出IgM+B细胞后，c-fos RNA变得无法检测到。前B细胞中c-fos的表达依赖于IL-7。然而，当在无IL-7的条件下培养6小时的前B细胞用IL-7重新刺激时，c-fos RNA并未被诱导。在重新刺激的前B细胞中，c-jun RNA的表达略有诱导。junB和junD RNA在细胞中处于稳态水平。这些基因产物在前B细胞中形成AP-1分子。这些结果表明，AP-1在依赖IL-7的前B细胞发育中起作用。
