利用酿酒酵母的基因探针鉴定巴氏酵母的新染色体。

Identification of new chromosomes of Saccharomyces bayanus using gene probes from S. cerevisiae.

作者信息

Naumov G I, Naumova E S, Gaillardin C, Turakainen H, Korhola M

机构信息

State Institute for Genetics and Selection of Industrial Microorganisms, Moscow, Russia.

出版信息

Hereditas. 1994;120(2):121-6. doi: 10.1111/j.1601-5223.1994.00121.x.

DOI:10.1111/j.1601-5223.1994.00121.x

PMID:8083058

Abstract

The Saccharomyces cerevisiae genes HIS3 (chr. XV), LYS2 (chr. II), ARG4 (chr. VIII), MF alpha 1 (chr. XVI) and MEL1 (chr. II) and the S. pastorianus gene MELx were used for identification of the homeologous chromosomes in S. bayanus by using pulsed-field gel electrophoresis and Southern blot hybridization. The results showed differences in hybridization intensities and mobilities between the homeologous chromosomes of the two sibling yeasts S. cerevisiae and S. bayanus. The probes HIS3, LYS2, MEL1, and MF alpha 1 identified four new homeologous chromosomes of S. bayanus. The ARG4 probe confirmed the previously identified location of chr. VIII (NAUMOV et al. 1992b). Using the MF alpha 1 gene, another chromosome besides chr. XVI was identified in some S. bayanus strains, suggesting translocation of this sequence to the S. bayanus chromosome that normally migrates to the position of chr. XI of S. cerevisiae.

摘要

酿酒酵母基因HIS3（第十五号染色体）、LYS2（第二号染色体）、ARG4（第八号染色体）、MFα1（第十六号染色体）和MEL1（第二号染色体）以及巴斯德酵母基因MELx，通过脉冲场凝胶电泳和Southern印迹杂交用于鉴定贝酵母中的同源染色体。结果显示，酿酒酵母和贝酵母这两个姊妹酵母的同源染色体之间在杂交强度和迁移率上存在差异。探针HIS3、LYS2、MEL1和MFα1鉴定出了贝酵母的四条新同源染色体。ARG4探针证实了先前确定的第八号染色体的位置（瑙莫夫等人，1992b）。使用MFα1基因，在一些贝酵母菌株中除了第十六号染色体外还鉴定出了另一条染色体，这表明该序列易位到了贝酵母染色体上，该染色体通常迁移到酿酒酵母第十一号染色体的位置。