Laverriere A C, MacNeill C, Mueller C, Poelmann R E, Burch J B, Evans T
Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111.
J Biol Chem. 1994 Sep 16;269(37):23177-84.
We describe experiments which show that: 1) two previously identified members of the GATA family of transcription factors (both of which were originally called GATA-4) correspond to chicken cDNAs for two distinct factors (which we now refer to as cGATA-4 and cGATA-5); 2) another new member of this family corresponds to a third factor designated cGATA-6; 3) each of these mRNAs displays a differential expression pattern. The cGATA-5 gene is initially transcribed in the cardiac crescent prior to formation of the primordial heart tube. Following formation of the primitive heart, cGATA-5 transcripts are evident in both endocardium and myocardium as well as in other lateral plate derivatives. The cGATA-5 gene is also transcribed in the primitive embryonic gut and in late stage embryos is sequentially up-regulated in distinct segments of gastrointestinal epithelia as they undergo terminal differentiation. These studies thus provide novel insights into tissue-specific regulation by GATA-5, as well as into possibly overlapping regulatory functions for these three family members.
我们描述了一些实验，这些实验表明：1）转录因子GATA家族中两个先前已鉴定的成员（最初都称为GATA-4）对应于两种不同因子的鸡cDNA（我们现在将其称为cGATA-4和cGATA-5）；2）该家族的另一个新成员对应于第三个因子，命名为cGATA-6；3）这些mRNA中的每一个都表现出差异表达模式。cGATA-5基因最初在原始心管形成之前的心脏新月中被转录。原始心脏形成后，cGATA-5转录本在内皮和心肌以及其他侧板衍生物中都很明显。cGATA-5基因也在原始胚胎肠道中被转录，在晚期胚胎中，随着胃肠道上皮细胞经历终末分化，它在不同节段中依次上调。因此，这些研究为GATA-5的组织特异性调控以及这三个家族成员可能重叠的调控功能提供了新的见解。
