Masuzawa T, Miyaura C, Onoe Y, Kusano K, Ohta H, Nozawa S, Suda T
Department of Biochemistry, School of Dentistry, Showa University, Tokyo, Japan.
J Clin Invest. 1994 Sep;94(3):1090-7. doi: 10.1172/JCI117424.
We have found that an estrogen deficiency causes a marked increase in bone marrow cells. To examine the effect of estrogen on hemopoiesis, we characterized the increased population of bone marrow cells after ovariectomy (OVX). In OVX mice, the percentage of myeloid cells and granulocytes was decreased, whereas that of B220-positive B lymphocytes was selectively increased 2-4 wk after surgery. The total number of myeloid cells and granulocytes did not change appreciably, but that of B220-positive cells was greatly increased by OVX. When OVX mice were treated with estrogen, the increased B lymphopoiesis returned to normal. B220-positive cells were classified into two subpopulations, B220low and B220high. The majority of the B220low cells were negative for the IgM mu chain, whereas most of the B220high cells were mu-positive. OVX selectively increased the precursors of B lymphocytes identified by B220low. mu-negative phenotype, suggesting that an estrogen deficiency stimulates accumulation of B lymphocyte precursors. When bone marrow-derived stromal cells (ST2) were pretreated with estrogen then co-cultured with bone marrow cells in the presence of estrogen, the stromal cell-dependent B lymphopoiesis was greatly inhibited. The present study suggests that estrogen plays an important role in the regulation of B lymphocyte development in mouse bone marrow.
我们发现雌激素缺乏会导致骨髓细胞显著增加。为了研究雌激素对造血的影响，我们对卵巢切除（OVX）后骨髓细胞数量增加的情况进行了表征。在OVX小鼠中，骨髓细胞和粒细胞的百分比降低，而B220阳性B淋巴细胞的百分比在手术后2 - 4周选择性增加。骨髓细胞和粒细胞的总数没有明显变化，但OVX使B220阳性细胞的数量大幅增加。当给OVX小鼠注射雌激素后，增加的B淋巴细胞生成恢复正常。B220阳性细胞分为两个亚群，B220low和B220high。大多数B220low细胞的IgMμ链呈阴性，而大多数B220high细胞的μ链呈阳性。OVX选择性增加了由B220low、μ链阴性表型鉴定的B淋巴细胞前体，表明雌激素缺乏会刺激B淋巴细胞前体的积累。当骨髓来源的基质细胞（ST2）用雌激素预处理，然后在有雌激素的情况下与骨髓细胞共培养时，基质细胞依赖的B淋巴细胞生成受到极大抑制。本研究表明雌激素在小鼠骨髓中B淋巴细胞发育的调节中起重要作用。
