Tabassum S, Shears P, Hart C A
Department of Medical Microbiology, University of Liverpool, United Kingdom.
J Med Virol. 1994 May;43(1):50-6. doi: 10.1002/jmv.1890430110.
Faecal samples were obtained from 111 children hospitalized with acute watery gastroenteritis in a children's hospital in Bangladesh from January to December, 1989. Rotavirus was detected as the aetiologic agent in 35 (32%) of these patients. The electrophoretic pattern of ds-RNA extracted from the rotaviruses showed 11 different migration patterns (six short and five long), as well as some short and long mixed profiles. All four major G serotypes were identified by amplification of a segment of the gene for VP7 using the polymerase chain reaction. A specific serotype could be assigned in 32 (91%) cases. Serotypes G1-G4 accounted for 11%, 37%, 11%, and 23% of isolates respectively. Three (9%) mixed serotypes were identified by this method and were found to be mixtures of serotypes G1 and G4, G2 and G4, and G3 and G4.
1989年1月至12月期间，从孟加拉国一家儿童医院111名因急性水样肠胃炎住院的儿童中采集粪便样本。在其中35名（32%）患者中检测到轮状病毒为病原体。从轮状病毒中提取的双链RNA的电泳图谱显示出11种不同的迁移模式（6种短型和5种长型），以及一些短型和长型混合图谱。通过聚合酶链反应扩增VP7基因片段，鉴定出所有四种主要的G血清型。在32例（91%）病例中可以确定特定血清型。血清型G1 - G4分别占分离株的11%、37%、11%和23%。通过该方法鉴定出3种（9%）混合血清型，发现它们是血清型G1和G4、G2和G4以及G3和G4的混合物。
