斯洛伐克与儿童腹泻相关的A组轮状病毒的血清型特征分析

Serotypic characterization of group A rotaviruses associated with children's diarrhea in Slovakia.

作者信息

Tietzová J, Petrovicová A

机构信息

Institute of Preventive and Clinical Medicine, 833 01 Bratislava, Slovakia.

出版信息

Folia Microbiol (Praha). 2000;45(2):183-6. doi: 10.1007/BF02817421.

DOI:10.1007/BF02817421

PMID:11271830

Abstract

A total of 368 rotavirus RNA-positive (PAGE) stool samples collected continually during 1992-95 from infants and young children under five years of age hospitalized with acute gastroenteritis were serotyped using an enzyme immunoassay with VP7-specific monoclonal antibodies (ELISA with MAbs) for serotypes G1-G4. The serotype was identified in 106 stool samples (29%). Comparison of electropherotype and serotype profile in individual samples did not show any remarkable correlation. The members of three electropherotypes (A, C, K) belonged to all 4 serotypes. The representatives of two electropherotypes (E, G) and of mixed electropherotype did not react with any of the specific monoclonal antibodies used. The distribution of the serotypes was scored as 52, 13, 14 and 7.5% for G1 G4, respectively, whereas 13% of samples reacted with two or more type-specific monoclonal antibodies. The G1 serotype dominated during the period followed.

摘要

1992年至1995年期间，持续收集了368份轮状病毒RNA阳性（聚丙烯酰胺凝胶电泳）粪便样本，这些样本来自因急性肠胃炎住院的5岁以下婴幼儿。使用针对G1 - G4血清型的VP7特异性单克隆抗体的酶免疫测定法（单克隆抗体酶联免疫吸附测定法）对这些样本进行血清分型。在106份粪便样本（29%）中鉴定出了血清型。对单个样本的电泳型和血清型谱进行比较，未显示出任何显著相关性。三种电泳型（A、C、K）的成员属于所有4种血清型。两种电泳型（E、G）的代表以及混合电泳型与所使用的任何特异性单克隆抗体均无反应。血清型的分布分别为G1型占52%、G2型占13%、G3型占14%、G4型占7.5%，而13%的样本与两种或更多种型特异性单克隆抗体发生反应。在随后的时期，G1血清型占主导地位。