Boniotti B, Wirblich C, Sibilia M, Meyers G, Thiel H J, Rossi C
Istituto Zooprofilattico Sperimentale della Lombardia e dell' Emilia, Brescia, Italy.
J Virol. 1994 Oct;68(10):6487-95. doi: 10.1128/JVI.68.10.6487-6495.1994.
Expression studies conducted in vitro and in Escherichia coli led to the identification of a protease from rabbit hemorrhagic disease virus (RHDV). The gene coding for this protease was found to be located in the central part of the genome preceding the putative RNA polymerase gene. It was demonstrated that the protease specifically cuts RHDV polyprotein substrates both in cis and in trans. Site-directed mutagenesis experiments revealed that the RHDV protease closely resembles the 3C proteases of picornaviruses with respect to the amino acids directly involved in the catalytic activity as well as to the role played by histidine as part of the substrate binding pocket.
在体外和大肠杆菌中进行的表达研究，促成了兔出血症病毒（RHDV）一种蛋白酶的鉴定。编码这种蛋白酶的基因位于基因组的中部，在假定的RNA聚合酶基因之前。已证明该蛋白酶能在顺式和反式条件下特异性切割RHDV多聚蛋白底物。定点诱变实验表明，RHDV蛋白酶在直接参与催化活性的氨基酸以及组氨酸作为底物结合口袋一部分所起的作用方面，与微小核糖核酸病毒的3C蛋白酶极为相似。
