Boyd A J, Sherman I A, Saibil F G
Aron M. Rappaport Microcirculation Laboratory, Sunnybrook Health Science Centre, Toronto, Ontario, Canada.
Microvasc Res. 1994 May;47(3):355-68. doi: 10.1006/mvre.1994.1028.
Polymorphonuclear leukocytes are thought to play a pivotal role in the generation of ischemia-reperfusion-induced mucosal injury; however, their behavior in the intestinal microvasculature following this injury has not been directly examined. In this study intravital microscopy was used to investigate the dynamics of blood flow and leukocyte behavior in the villus, serosal, and mesenteric microcirculation during ischemia-reperfusion in anesthetized hamsters. Thirty minutes of ischemia and 90 min of reperfusion resulted in almost complete microvascular stasis in the villi of control animals, whereas only a few serosal and mesenteric microvessels exhibited stasis. Following reperfusion, there was a significant increase in leukocyte accumulation in all three tissues; however, significantly fewer leukocytes adhered in the villus microvasculature than in the rest of either the mucosa, serosa, or mesentery. Treatment with gamma-hydroxybutyrate (GHB), a compound that we have previously demonstrated to be highly effective in preventing ischemia-reperfusion injury, significantly reduced both the microvascular stasis and leukocyte accumulation in all three vascular beds. This study demonstrates that there are significant differences in the response to ischemia-reperfusion in various intestinal layers and that areas most susceptible to damage are not necessarily those exhibiting the greatest leukocyte accumulation.
多形核白细胞被认为在缺血再灌注诱导的黏膜损伤的发生中起关键作用；然而，这种损伤后它们在肠道微血管中的行为尚未得到直接研究。在本研究中，活体显微镜被用于研究麻醉的仓鼠在缺血再灌注期间绒毛、浆膜和肠系膜微循环中的血流动力学和白细胞行为。30分钟的缺血和90分钟的再灌注导致对照动物绒毛中的微血管几乎完全停滞，而只有少数浆膜和肠系膜微血管出现停滞。再灌注后，所有三个组织中的白细胞聚集均显著增加；然而，绒毛微血管中黏附的白细胞明显少于黏膜、浆膜或肠系膜其他部位。用γ-羟基丁酸（GHB）治疗，我们之前已证明该化合物在预防缺血再灌注损伤方面非常有效，显著减少了所有三个血管床中的微血管停滞和白细胞聚集。本研究表明，不同肠层对缺血再灌注的反应存在显著差异，最易受损的区域不一定是白细胞聚集最多的区域。
