Kinouchi H, Sharp F R, Chan P H, Koistinaho J, Sagar S M, Yoshimoto T
Division of Neurosurgery, Institute of Brain Diseases, Tohoku University School of Medicine, Sendai, Japan.
Neurosci Lett. 1994 Apr 25;171(1-2):163-6. doi: 10.1016/0304-3940(94)90630-0.
Middle cerebral artery (MCA) occlusion in halothane-anesthetized rats induced the zinc finger gene, NGFI-A, in brain. In situ hybridization studies showed that NGFI-A was induced throughout all of the cortex following MCA occlusion. By 24 h after MCA occlusion there was little expression of NGFI-A mRNA in the core of the MCA infarct, but the mRNA was still induced in all of cortex outside the infarct. MCA occlusion also induced this gene in subcortical structures: ipsilateral medial striatum; most of thalamus including medial and lateral geniculate nuclei; substantia nigra; and hippocampus at 4 h of MCA occlusion which generally disappeared by 24 h of MCA occlusion. Most of these structures, except for the striatum, are not supplied by the MCA. These data show that changes in brain gene expression can occur in many regions remote from an infarction.
在氟烷麻醉的大鼠中，大脑中动脉（MCA）闭塞可诱导大脑中的锌指基因NGFI-A。原位杂交研究表明，MCA闭塞后，整个皮质均诱导产生NGFI-A。MCA闭塞后24小时，MCA梗死核心区域几乎没有NGFI-A mRNA表达，但梗死灶外的整个皮质中仍可诱导产生该mRNA。MCA闭塞还可在皮质下结构中诱导该基因：同侧内侧纹状体；包括内侧和外侧膝状体核在内的大部分丘脑；黑质；以及MCA闭塞4小时时的海马体，到MCA闭塞24小时时这些通常消失。除纹状体外，这些结构中的大多数并非由MCA供血。这些数据表明，脑梗死远处的许多区域均可发生脑基因表达变化。
