Naoi M, Maruyama W, Dostert P
Department of Biosciences, Nagoya Institute of Technology, Japan.
Neurosci Lett. 1994 Apr 25;171(1-2):9-12. doi: 10.1016/0304-3940(94)90591-6.
1,2(N)-Dimethyl-6,7-dihydroxy-isoquinolinium ion (N-methylsalsolinium ion) was found to bind to melanin, while N-methyl(R)salsolinol and (R)salsolinol did not. Two components with different affinity to melanin were found; the dissociation constant and maximal binding capacity were obtained as 2.1 and 79.4 nM, and 103 and 1336 pmol/mg melanin, respectively. The binding was enhanced by ferrous ion markedly and inhibited by 1-methyl-4-phenylpyridinium ion and ferric ion. N-Methylsalsolinium ion may be accumulated in dopamine neurons as melanin conjugate, which is regulated by ferric and ferrous ions.
发现1,2（N）-二甲基-6,7-二羟基异喹啉离子（N-甲基胡豆碱离子）能与黑色素结合，而N-甲基（R）胡豆醇和（R）胡豆醇则不能。发现了两种对黑色素具有不同亲和力的成分；解离常数和最大结合容量分别为2.1和79.4 nM，以及103和1336 pmol/mg黑色素。亚铁离子可显著增强这种结合，而1-甲基-4-苯基吡啶离子和铁离子则可抑制这种结合。N-甲基胡豆碱离子可能作为黑色素共轭物在多巴胺神经元中积累，这受铁离子和亚铁离子的调节。
