Brambilla R, Draetta G
European Molecular Biology Laboratory, Heidelberg, Germany.
Oncogene. 1994 Oct;9(10):3037-41.
Several members of the cdk family of protein kinases are involved in the regulation of the eukaryotic cell cycle. Using a PCR-based strategy we have screened different human tumor cell lines for cdk-related cDNAs. One clone isolated from the bladder carcinoma cell line RT112 encodes a novel protein kinase named PISSLRE, based on its predicted sequence at the conserved PSTAIRE motif. PISSLRE showed 50% amino acid identity with the previously isolated p58KGTA. PISSLRE contained all the structural elements featured by cyclin dependent kinases, including a proline in the PSTAIRE motif, which might be important for cyclin binding. PISSLRE was found expressed as 2.0 kb messenger RNA in a variety of human cell lines. Its expression was not restricted to tumor cells as it was detectable also in normal fibroblasts. In adult tissues, PISSLRE mRNA showed the highest expression in lung, liver and kidney. The broad expression pattern in adult tissues might suggest that PISSLRE could be involved in processes distinct from cell proliferation.
蛋白激酶cdk家族的几个成员参与真核细胞周期的调控。我们采用基于聚合酶链反应（PCR）的策略，在不同的人类肿瘤细胞系中筛选与cdk相关的互补脱氧核糖核酸（cDNA）。从膀胱癌细胞系RT112中分离出的一个克隆编码一种新的蛋白激酶，根据其在保守的PSTAIRE基序处的预测序列，将其命名为PISSLRE。PISSLRE与先前分离出的p58KGTA有50%的氨基酸同一性。PISSLRE包含细胞周期蛋白依赖性激酶的所有结构元件，包括PSTAIRE基序中的一个脯氨酸，这可能对细胞周期蛋白结合很重要。在多种人类细胞系中发现PISSLRE以2.0 kb的信使核糖核酸（mRNA）形式表达。其表达并不局限于肿瘤细胞，在正常成纤维细胞中也可检测到。在成人组织中，PISSLRE mRNA在肺、肝和肾中表达最高。在成人组织中的广泛表达模式可能表明PISSLRE可能参与了与细胞增殖不同的过程。
