Singer L K, Ritter S
Department of Veterinary and Comparative Anatomy, Pharmacology, and Physiology, Washington State University, Pullman 99164-6520.
Physiol Behav. 1994 Jul;56(1):193-6. doi: 10.1016/0031-9384(94)90279-8.
We examined the effects of glucose, lipid, or fructose infusion on 2-deoxy-D-glucose (2DG)- and 2-mercaptoacetate (MA)-induced feeding. Rats with chronic intra-atrial catheters were infused for 15 min with one of the above nutrients and then injected with 2DG or MA. The nutrient infusion continued for the next 2 h and food intake was simultaneously measured. For control conditions, equitonic saline was infused, and 2DG, MA, or saline was injected. We found that glucose was the only nutrient source capable of significantly reducing 2DG-induced feeding. Equicaloric infusions of lipid or fructose did not significantly reduce 2DG-induced feeding. However, feeding induced by MA was similarly and significantly reduced by glucose, lipid, and fructose. These results suggest that 2DG-induced feeding is mediated by receptor cells that are selectively responsive to glucose availability, and MA-induced feeding is mediated by receptor cells that are responsive to a wider range of metabolic fuels.
我们研究了输注葡萄糖、脂质或果糖对2-脱氧-D-葡萄糖（2DG）和2-巯基乙酸（MA）诱导进食的影响。给植入慢性心房导管的大鼠输注上述营养素之一15分钟，然后注射2DG或MA。接下来的2小时继续输注营养素，并同时测量食物摄入量。作为对照条件，输注等渗盐水，并注射2DG、MA或盐水。我们发现，葡萄糖是唯一能够显著减少2DG诱导进食的营养源。等热量输注脂质或果糖并没有显著减少2DG诱导的进食。然而，葡萄糖、脂质和果糖对MA诱导的进食有相似且显著的减少作用。这些结果表明，2DG诱导的进食是由对葡萄糖可用性有选择性反应的受体细胞介导的，而MA诱导的进食是由对更广泛代谢燃料有反应的受体细胞介导的。
