Liu S Y, Zhang W Y, Li H Y, Dai X J, Zhang Y, Xu J C, Chen L L, Wang S Q, Qian C H
Shanghai Institute of Organic Chemistry, Academia Sinica.
Sheng Li Xue Bao. 1994 Feb;46(1):83-9.
The present study was undertaken to explore endogenous sleep factors isolated from 48-72 h sleep deprived (SD) male Tupaia belangeri chinensis (TBC). Only drink ad libitum (10% glucose) was available within 24 h before collection of urine. Controlled "clean" urinary samples were pooled and stored at 20 C. Fraction I-V from the urine were determined after ultrafiltration and Sephadex-G15. Amino-acid analysis of each fraction was automatically done by a 835 Amino-acid Analyzer, respectively. Bioassay was performed in 40 adult rabbits weighing 2.5-3.5 kg of either sex. Experiments were undertaken via the mesodiencephalic intraventricular infusion. Results show that S2C (Fraction-III) (50 micrograms/rabbit, i.c.v.) exhibited significant delta-enhancing effect compared to the controls. Further purification was done with Sephadex G-25 and Sephadex LH-20. The more purified S4B (50 micrograms/rabbit, i.c.v.) also exhibited significant delta-enhancing effect compared to the controls. The amino-acid analysis of Fraction-III revealed that the compositional contents of S2C and S4B are different from what have been known with Factor S, DSIP and SPS.
本研究旨在探索从48 - 72小时睡眠剥夺（SD）的雄性树鼩（Tupaia belangeri chinensis，TBC）中分离出的内源性睡眠因子。在收集尿液前24小时内，仅随意提供饮水（10%葡萄糖）。将经过控制的“清洁”尿液样本汇集并储存在20℃。尿液的I - V组分在超滤和Sephadex - G15后进行测定。每个组分的氨基酸分析分别由835型氨基酸分析仪自动完成。对40只体重在2.5 - 3.5千克、雌雄不限的成年兔子进行生物测定。实验通过中脑间脑室内注射进行。结果显示，与对照组相比，S2C（组分III）（50微克/只兔子，脑室内注射）表现出显著的增强δ波的作用。使用Sephadex G - 25和Sephadex LH - 20进行进一步纯化。与对照组相比，纯化程度更高的S4B（50微克/只兔子，脑室内注射）也表现出显著的增强δ波的作用。对组分III的氨基酸分析表明，S2C和S4B的组成成分与已知的S因子、DSIP和SPS不同。
