Schoenhard G L, Bishop P E, Lee D J, Sinnhuber R O
J Assoc Off Anal Chem. 1975 Sep;58(5):1074-6.
A rapid and sensitive microbial assay was developed to detect lethal products of aflatoxin B metabolism by rainbow trout (Salmon gairdneri) Mt. Shasta strain. Bacillus subtilis GSY 1057 (hisA1, uvr-1, metB4), a DNA repair deficient strain, was incubated for 20 min in the 20,000 times g supernate from trout liver homogenates which had been preincubated for 10 min with various levels of aflatoxin B. Serial dilutions of the incubation mixture were plated in triplicate on tryptose blood agar base plates and colonies were counted after 12 hr at 37 degrees C. One mumole aflatoxin B in 3.2 ml incubation mixture reduced viability 60%.
开发了一种快速灵敏的微生物检测方法，用于检测虹鳟（Salmon gairdneri）沙斯塔山菌株对黄曲霉毒素B代谢产生的致死产物。将枯草芽孢杆菌GSY 1057（hisA1，uvr-1，metB4），一种DNA修复缺陷菌株，在来自鳟鱼肝匀浆的20000倍重力超速离心上清液中孵育20分钟，该上清液已与不同水平的黄曲霉毒素B预孵育10分钟。将孵育混合物的系列稀释液一式三份接种在胰蛋白胨血琼脂基础平板上，在37℃下培养12小时后计数菌落。3.2毫升孵育混合物中1微摩尔黄曲霉毒素B使活力降低60%。
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