腹泻性贝类毒素检测方法的比较

A comparison of methods for diarrhoeic shellfish poison detection.

作者信息

Gucci P M, Serse A P, Coccia A M, Tubaro A, Della Loggia R, Gianna R, Bruno M, Volterra L

机构信息

Laboratory of Environmental Hygiene-Istituto Superiore di Sanità, Rome, Italy.

出版信息

Toxicol Lett. 1994 Oct;74(1):91-7. doi: 10.1016/0378-4274(94)90077-9.

DOI:10.1016/0378-4274(94)90077-9

PMID:8085273

Abstract

Samples of diarrhoeic shellfish poison (DSP) mussels from several parts of the Italian Adriatic coastline were extracted and tested according to a number of different methods presently available, i.e. Yasumoto's mouse biotest, Kat's biotest, the ELISA test and the HPLC method. Results were compared for toxic levels detected in each sample. While a common qualitative result (toxic/non-toxic) was given by all the methods, no clear quantitative agreement was found. The differences between methods and consequent lack of agreement in results are discussed.

摘要

从意大利亚得里亚海沿岸几个地区采集的腹泻性贝类毒素（DSP）贻贝样本，按照目前可用的多种不同方法进行了提取和检测，即矢本小鼠生物试验、卡特生物试验、酶联免疫吸附测定（ELISA）试验和高效液相色谱（HPLC）法。对每个样本中检测到的毒素水平的结果进行了比较。虽然所有方法给出了相同的定性结果（有毒/无毒），但未发现明确的定量一致性。讨论了方法之间的差异以及结果缺乏一致性的情况。