Brewer J M, Alexander J
Department of Immunology, Todd Centre, University of Strathclyde, Glasgow, UK.
Vaccine. 1994 May;12(7):613-9. doi: 10.1016/0264-410x(94)90265-8.
The ability of non-ionic surfactant vesicles (NISV) to stimulate humoral responses to bovine serum albumin (BSA) in H-2b, H-2d and H-2k congenic mice on the Balb genetic background was compared with that of Freund's complete adjuvant (FCA). After two subcutaneous inoculations of BSA formulated in each adjuvant, the NISV preparation was found to stimulate significantly higher total antibody production than FCA in mice carrying the H-2b haplotype at all time points measured after secondary inoculation (2, 5 and 10 weeks) and in Balb/c mice (H-2d) at two weeks after inoculation. Both adjuvants were found to overcome the apparent non-responsiveness of Balb/B mice (H-2b) to BSA alone. Analysis of the IgG subclass responses to BSA revealed a pattern of IgG1 but not IgG2a production similar to that for whole immunoglobulin. IgG1 responses invariably differed significantly, not only between adjuvant formulations but also between different H-2 haplotypes receiving the same inoculation. On the other hand, IgG2a responses did not differ significantly between H-2 haplotypes in animals given the same adjuvant preparations, although they did differ significantly in mice given BSA alone. Therefore, these results suggest that adjuvants cannot only circumvent antigen-specific non-responsiveness or low responsiveness, but also can induce antibody isotype switching independent of major histocompatibility complex controls.
将非离子表面活性剂囊泡（NISV）与弗氏完全佐剂（FCA）相比较，观察它们在Balb遗传背景下的H-2b、H-2d和H-2k同源小鼠中刺激对牛血清白蛋白（BSA）产生体液免疫反应的能力。在分别用每种佐剂配制的BSA进行两次皮下接种后，发现在二次接种后所有测量时间点（2、5和10周）携带H-2b单倍型的小鼠以及接种后两周的Balb/c小鼠（H-2d）中，NISV制剂刺激产生的总抗体量显著高于FCA。两种佐剂均能克服Balb/B小鼠（H-2b）对单独BSA明显的无反应性。对BSA的IgG亚类反应分析显示，IgG1而非IgG2a的产生模式与全免疫球蛋白相似。IgG1反应不仅在佐剂制剂之间，而且在接受相同接种的不同H-2单倍型之间始终存在显著差异。另一方面，在给予相同佐剂制剂的动物中，H-2单倍型之间的IgG2a反应没有显著差异，尽管在单独给予BSA的小鼠中它们确实存在显著差异。因此，这些结果表明，佐剂不仅可以规避抗原特异性无反应性或低反应性，而且还可以诱导抗体同种型转换，而不受主要组织相容性复合体的控制。
