Mitchell L A, Décarie D, Tingle A J, Lacroix M, Zrein M
Department of Pathology, University of British Columbia, Vancouver, Canada.
Vaccine. 1994 May;12(7):639-45. doi: 10.1016/0264-410x(94)90270-4.
Synthetic peptides (SPs), 18-29 amino acids long, representing selected sequences of rubella virus (RV) capsid (C) protein were used in lymphocyte proliferation assays to identify antigenic regions recognized by T lymphocytes from healthy RV-reactive adults. Four SPs, C(1-29), C(90-114), C(108-134) and C(255-300), stimulated proliferation of peripheral blood mononuclear cells and RV-specific T-cell lines from the same donors. C(1-29V), an SP analogue containing an RA27/3 RV vaccine strain sequence, stimulated higher levels of proliferation in T cells obtained from RV-vaccinated subjects than did the comparable wild-type (M33 strain) RV sequence.
使用长度为18 - 29个氨基酸的合成肽（SPs），其代表风疹病毒（RV）衣壳（C）蛋白的选定序列，用于淋巴细胞增殖试验，以鉴定健康的RV反应性成年人T淋巴细胞识别的抗原区域。四种SPs，即C(1 - 29)、C(90 - 114)、C(108 - 134)和C(255 - 300)，刺激了来自相同供体的外周血单核细胞和RV特异性T细胞系的增殖。C(1 - 29V)是一种含有RA27/3 RV疫苗株序列的SP类似物，与可比的野生型（M33株）RV序列相比，它刺激了从接种RV疫苗的受试者获得的T细胞中更高水平的增殖。
