Ilonen J, Seppänen H, Närvänen A, Korkolainen M, Salmi A A
Department of Virology, University of Turku, Finland.
Viral Immunol. 1992 Fall;5(3):221-8. doi: 10.1089/vim.1992.5.221.
Antigenicity of rubella virus E1 polypeptide was analyzed using synthetic peptides with predicted amino acid sequences. Overlapping solid-phase bound peptides were used to define antibody binding domains and a panel of free peptides to study T-cell responsiveness. Several antibody-binding areas including those earlier described to contain major neutralizing epitopes were recognized by human sera positive for rubella antibodies. T-cell lines specific for rubella virus were established from 14 rubella immune subjects. All cell lines responded to rubella virion-derived antigen but only eight (57%) responded to one or more of the synthetic peptides. Individual patterns of peptide recognition were found but peptide 8 representing amino acids 402-422 was most often stimulatory to T-cells lines, either alone (3 subjects) or in combination with peptide 3 (amino acids 245-269) or 3 and 4 (amino acids 269-287). The response was HLA restricted but no single DR specificity for this restriction was identified.
利用具有预测氨基酸序列的合成肽分析风疹病毒E1多肽的抗原性。使用重叠的固相结合肽来确定抗体结合域，并使用一组游离肽来研究T细胞反应性。风疹抗体阳性的人血清识别出几个抗体结合区域，包括那些先前描述为含有主要中和表位的区域。从14名风疹免疫受试者中建立了风疹病毒特异性T细胞系。所有细胞系都对风疹病毒体衍生的抗原产生反应，但只有8个（57%）对一种或多种合成肽产生反应。发现了个体的肽识别模式，但代表氨基酸402 - 422的肽8最常刺激T细胞系，单独（3名受试者）或与肽3（氨基酸245 - 269）或肽3和4（氨基酸269 - 287）组合时均可。该反应受HLA限制，但未确定这种限制的单一DR特异性。
