McSweeney C S, Dulieu A, Katayama Y, Lowry J B
Long Pocket Laboratories, CSIRO Division of Tropical Animal Production, Indooroopilly, Queensland, Australia.
Appl Environ Microbiol. 1994 Aug;60(8):2985-9. doi: 10.1128/aem.60.8.2985-2989.1994.
The ability of the ruminal anaerobic phycomycete Neocallimastix patriciarum to digest model lignin compounds and lignified structures in plant material was studied in batch culture. The fungus did not degrade or transform model lignin compounds that were representative of the predominant intermonomer linkages in lignin, nor did it solubilize acid detergent lignin that had been isolated from spear grass. In a stem fraction of sorghum, 33.6% of lignin was apparently solubilized by the fungus. Solubilization of ester- and either-linked phenolics accounted for 9.2% of the lignin released. The amounts of free phenolic acids detected in culture fluid were equivalent to the apparent loss of ester-linked phenolics from the sorghum substrate. However, the fungus was unable to cleave the ether bond in hydroxycinnamic acid bridges that cross-link lignin and polysaccharide. It is suggested that the majority of the solubilized lignin fraction was a lignin carbohydrate complex containing ether-linked hydroxycinnamic acids. The lignin carbohydrate complex was probably solubilized through dissolution of xylan in the lignin-xylan matrix rather than by lignin depolymerization.
在分批培养中研究了瘤胃厌氧藻状菌新美鞭菌消化植物材料中模型木质素化合物和木质化结构的能力。该真菌不会降解或转化代表木质素中主要单体间连接的模型木质素化合物，也不会溶解从矛草中分离出的酸性洗涤木质素。在高粱的茎部分，33.6%的木质素显然被该真菌溶解。酯键连接和醚键连接酚类物质的溶解占释放木质素的9.2%。在培养液中检测到的游离酚酸量相当于高粱底物中酯键连接酚类物质的明显损失。然而，该真菌无法裂解交联木质素和多糖的羟基肉桂酸桥中的醚键。有人认为，溶解的木质素部分大部分是含有醚键连接羟基肉桂酸的木质素碳水化合物复合物。木质素碳水化合物复合物可能是通过木聚糖在木质素-木聚糖基质中的溶解而溶解，而不是通过木质素解聚。
