Chen W, Supanwong K, Ohmiya K, Shimizu S, Kawakami H
Appl Environ Microbiol. 1985 Dec;50(6):1451-6. doi: 10.1128/aem.50.6.1451-1456.1985.
Veratrylglycerol-beta-guaiacyl ether (0.2 g/liter), a lignin model compound, was found to be degraded by mixed rumen bacteria in a yeast extract medium under strictly anaerobic conditions to the extent of 19% within 24 h. Guaiacoxyacetic acid, 2-(o-methoxyphenoxy)ethanol, vanillic acid, and vanillin were detected as degradation products of veratrylglycerol-beta-guaiacyl ether by thin-layer chromatography, gas chromatography, and gas chromatography-mass spectrometry. Guaiacoxyacetic acid (0.25 g/liter), when added into the medium as a substrate, was entirely degraded within 36 h, resulting in the formation of phenoxyacetic acid, guaiacol, and phenol. These results suggest that the beta-arylether bond, an important intermonomer linkage in lignin, can be cleaved completely by these rumen anaerobes.
藜芦基甘油-β-愈创木基醚（0.2克/升），一种木质素模型化合物，发现在严格厌氧条件下，于酵母提取物培养基中可被瘤胃混合细菌降解，24小时内降解程度达19%。通过薄层色谱、气相色谱和气相色谱-质谱法检测到藜芦基甘油-β-愈创木基醚的降解产物有愈创木氧基乙酸、2-（邻甲氧基苯氧基）乙醇、香草酸和香草醛。当将愈创木氧基乙酸（0.25克/升）作为底物添加到培养基中时，在36小时内完全降解，生成苯氧基乙酸、愈创木酚和苯酚。这些结果表明，木质素中重要的单体间连接键β-芳基醚键可被这些瘤胃厌氧菌完全裂解。
