MacDonald C, Vass M, Willett B, Scott A, Grant H
Department of Biological Sciences, University of Paisley, UK.
Hum Exp Toxicol. 1994 Jun;13(6):439-44. doi: 10.1177/096032719401300613.
The differentiated hepatic function of two rat liver cell lines, P9 and SV40RH1, immortalised by transfection with SV40 DNA has been investigated in terms of the glutathione synthesis, and the activities of gamma-glutamyltransferase, glutathione-S-transferase and UDP-glucuronosyltransferase. SV40RH1 is a highly differentiated cell line at early passage, but the expression of some aspects of its differentiated phenotype is unstable and some functions are lost by passage 12-13. P9 is a less-well differentiated cell line, with relatively stable expression of functions between passages 4 and 13. In terms of differentiated function both cell lines represent a marked improvement over primary cultures of rat hepatocytes which de-differentiate rapidly within 24-48 h in culture. This retention of liver function in proliferating cell lines offers the opportunity to use such cells in in vitro toxicological studies.
通过用SV40 DNA转染永生化的两种大鼠肝细胞系P9和SV40RH1的分化肝功能，已根据谷胱甘肽合成以及γ-谷氨酰转移酶、谷胱甘肽-S-转移酶和UDP-葡萄糖醛酸基转移酶的活性进行了研究。SV40RH1在传代早期是一种高度分化的细胞系，但其分化表型某些方面的表达不稳定，到第12 - 13代时一些功能丧失。P9是一种分化程度较低的细胞系，在第4代到第13代之间功能表达相对稳定。就分化功能而言，这两种细胞系都比大鼠肝细胞原代培养有显著改善，后者在培养24 - 48小时内迅速去分化。增殖细胞系中肝功能的这种保留为在体外毒理学研究中使用此类细胞提供了机会。
