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SV40 序列转染永生化大鼠肝细胞。

The immortalisation of rat hepatocytes by transfection with SV40 sequences.

机构信息

University of Paisley, High Street, Paisley, PA1 2BE, Scotland.

出版信息

Cytotechnology. 1997 Jan;23(1-3):161-70. doi: 10.1023/A:1007907416596.

DOI:10.1023/A:1007907416596
PMID:22358532
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3449864/
Abstract

Abbreviations EGTA - ethylene bis(oxyethylenenitrilo)-tetraacetic acid; F12 - Ham's F12; FBS - foetal bovine serum; HBSS - Hank's balanced salt solution; HDM - hormonally defined medium; HEPES - 4-(2-hydroxyethyl)-1-piperazine ethanesulphonic acid; NBS - new born calf serum; WME - Williams' medium E.

摘要

缩写 EGTA- 乙二胺四乙酸; F12- 汉氏 F12; FBS- 胎牛血清; HBSS- 汉克平衡盐溶液; HDM- 激素定义的培养基; HEPES- 4-(2-羟乙基)-1-哌嗪乙磺酸; NBS- 新生小牛血清; WME- 威廉姆斯介质 E。

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本文引用的文献

1
Altered expression of cytochrome P-450 mRNAs, and potentially of other transcripts encoding key hepatic functions, are triggered during the isolation of rat hepatocytes.在大鼠肝细胞分离过程中，细胞色素P - 450 mRNA以及可能编码关键肝功能的其他转录本的表达会发生改变。
Biochem J. 1993 Feb 1;289 ( Pt 3)(Pt 3):621-4. doi: 10.1042/bj2890621.
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Expression of liver functions in immortalised rat hepatocyte cell lines.永生化大鼠肝细胞系中肝功能的表达
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3
Establishment and characterization of murine macrophage-like cell lines following transformation with simian virus 40 DNA deleted at the origin of replication.用在复制起点缺失的猿猴病毒40 DNA转化后建立并鉴定小鼠巨噬细胞样细胞系。
J Immunol Methods. 1994 Sep 14;174(1-2):33-51. doi: 10.1016/0022-1759(94)90008-6.
4
Simian virus 40 large tumor antigen-immortalized normal human liver epithelial cells express hepatocyte characteristics and metabolize chemical carcinogens.猿猴病毒40大T抗原永生化的正常人肝上皮细胞表达肝细胞特征并代谢化学致癌物。
Proc Natl Acad Sci U S A. 1993 Jun 1;90(11):5123-7. doi: 10.1073/pnas.90.11.5123.
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Immortalization of epithelial-like cells from human liver tissue with SV40 T-antigen gene.利用SV40 T抗原基因使人肝脏组织中的上皮样细胞永生化。
Exp Cell Res. 1993 May;206(1):27-35. doi: 10.1006/excr.1993.1116.
6
Maintenance and reversibility of active albumin secretion by adult rat hepatocytes co-cultured with another liver epithelial cell type.与另一种肝上皮细胞类型共培养的成年大鼠肝细胞中活性白蛋白分泌的维持与可逆性。
Exp Cell Res. 1983 Jan;143(1):47-54. doi: 10.1016/0014-4827(83)90107-6.
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Hepatocyte proliferation in vitro: its dependence on the use of serum-free hormonally defined medium and substrata of extracellular matrix.体外肝细胞增殖：其对无血清激素限定培养基及细胞外基质底物使用的依赖性。
Proc Natl Acad Sci U S A. 1984 Mar;81(5):1411-5. doi: 10.1073/pnas.81.5.1411.
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High efficiency DNA-mediated transformation of primate cells.灵长类细胞的高效DNA介导转化
Science. 1983 Aug 5;221(4610):551-3. doi: 10.1126/science.6306768.
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Expression of early genes of origin-defective mutants of simian virus 40.猴病毒40起源缺陷型突变体早期基因的表达
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