Li W W, Shen W Z, Hung Y, Jen P Y, Yew D T
Department of Anatomy, Chinese University of Hong Kong, Shatin.
Eur J Morphol. 1994 Mar;32(1):49-57.
The infiltration of T lymphocytes and macrophages was examined in the retinae of traumatized and non-traumatized contra-lateral eyes of mice. Traumatization was produced by channeling a small hole in the limbic region of a single eye in each animal while it was under Nembutal anesthesia and a small piece of iron wire was inserted into the eye. After the T lymphocytes and macrophages were made apparent by immuno-histochemistry, they were counted on sectioned strips of the peripheral retinae 250 micrometer in length in both traumatized and non-traumatized eyes. During the first two weeks after the induced disturbance, both the retinae of the traumatized and non-traumatized eyes showed infiltration of T lymphocytes and macrophages. Although this increase in T lymphocyte infiltration was observed in both damaged eye and the undamaged one, the former displayed an enhanced increase. The contrary was noticed for macrophages infiltrating the undamaged eye. A bilateral response was therefore evident through the cellular infiltration in the traumatized and un-traumatized eyes. Morphometrical observations also revealed nuclear size changes in the retina during this acute phase of traumatization.
在小鼠受伤和未受伤的对侧眼睛视网膜中检测T淋巴细胞和巨噬细胞的浸润情况。在每只动物处于戊巴比妥麻醉状态时，通过在单眼的边缘区域钻一个小孔并插入一小段铁丝来造成损伤。在用免疫组织化学方法使T淋巴细胞和巨噬细胞显现后，对受伤和未受伤眼睛中长度为250微米的周边视网膜切片条进行计数。在诱发干扰后的前两周内，受伤和未受伤眼睛的视网膜均显示出T淋巴细胞和巨噬细胞的浸润。尽管在受损眼和未受损眼中均观察到T淋巴细胞浸润增加，但前者的增加更为明显。对于浸润未受损眼的巨噬细胞则观察到相反的情况。因此，通过受伤和未受伤眼睛中的细胞浸润可明显看出双侧反应。形态计量学观察还揭示了在创伤急性期视网膜细胞核大小的变化。
