Mackiewicz J, Szczesny P, Zagórski Z
Katedry i I Kliniki Okulistyki AM w Lublinie.
Klin Oczna. 1998;100(1):5-8.
To investigate the retinal toxicity of a vitreous substitute perfluorodecalin (PFD) in the rabbit eye up to 2 weeks after injection.
A space was created in the vitreous cavity by injecting 0.4 cc of perfluoropropane gas. After 3 days gas-fluid exchange was performed. Experimental eyes were injected with 1 cc of PFD and control eyes with 1 cc of Ringer solution. The eyes were enucleated 1, 3, 6 and 14 days after the procedure and histological studies were conducted.
Control samples showed almost normal histology. One day after PFD injection photoreceptor nuclei dropout and migration below the outer limiting membrane, pyknosis and occasional densification of cell bodies were observed. After 3 days glial cell hypertrophy and accumulation of macrophages above the inner limiting membrane was also noted. After 6 days necrosis was observed in the outer and inner retinal layers. Foci of cell death in the retina were also observed 14 days after PFD injection.
PFD induced degenerative changes in the rabbit retina within the first 24 hours after administration. Structural alterations in the inner and outer retina persisted within the two weeks of observation. Our results support the removal of PFD at the end of the primary surgical procedure.
研究玻璃体替代物全氟萘烷（PFD）在兔眼内注射后长达2周的视网膜毒性。
通过注射0.4 cc全氟丙烷气体在玻璃体腔中制造一个空间。3天后进行气液交换。实验眼注射1 cc PFD，对照眼注射1 cc林格液。术后1、3、6和14天摘除眼球并进行组织学研究。
对照样本显示组织学几乎正常。注射PFD后1天，观察到光感受器细胞核脱落并迁移至外限制膜下方，细胞体固缩且偶尔致密化。3天后还注意到胶质细胞肥大以及巨噬细胞在内限制膜上方积聚。6天后在外层和内层视网膜观察到坏死。注射PFD后14天也观察到视网膜中的细胞死亡灶。
PFD在给药后的最初24小时内诱导兔视网膜发生退行性变化。在观察的两周内，视网膜内层和外层的结构改变持续存在。我们的结果支持在初次手术结束时移除PFD。
