Tanigawa T, Robb L, Green A R, Begley C G
Walter and Eliza Hall Institute of Medical Research, Royal Melbourne Hospital, Victoria, Australia.
Cell Growth Differ. 1994 Jun;5(6):557-61.
The murine myeloid leukemia WEHI-3B contains an intracisternal A-particle insertion in a growth factor gene (IL-3) and a homeobox gene (Hox 2.4). Southern blot analysis of the differentiation unresponsive subline WEHI-3BD- demonstrated a rearrangement of the SCL gene, a gene that encodes a protein of the helix-loop-helix class of transcription factors. Northern blot analysis demonstrated an abnormal SCL transcript in the WEHI-3BD- cells, although SCL expression was not detected in the parental cells. The rearrangement was cloned and confirmed as an insertion of an intracisternal A-particle in the 3' untranslated region of the SCL gene. Although SCL is implicated in up to 25% of human T-cell acute lymphoblastic leukemia, this is the first example of SCL involvement in a myeloid leukemia.
小鼠髓性白血病WEHI-3B在一个生长因子基因（IL-3）和一个同源框基因（Hox 2.4）中含有一个池内A颗粒插入。对分化无反应的亚系WEHI-3BD-的Southern印迹分析显示SCL基因发生了重排，该基因编码一种螺旋-环-螺旋类转录因子的蛋白质。Northern印迹分析表明，在WEHI-3BD-细胞中存在异常的SCL转录本，尽管在亲代细胞中未检测到SCL表达。该重排被克隆并确认为池内A颗粒插入到SCL基因的3'非翻译区。尽管SCL与高达25%的人类T细胞急性淋巴细胞白血病有关，但这是SCL参与髓性白血病的首个实例。
