Johnson E A, Burke C N, Fredrickson T N, DiCapua R A
J Natl Cancer Inst. 1975 Jul;55(1):89-99. doi: 10.1093/jnci/55.1.89.
Three evaluative systems, immunodiffusion, fluorescent antibody (FA), and electron microscopy (EM), were used to follow the morphogenesis of Marek's disease virus in inoculated chickens. Of the three, EM and FA were the most sensitive in detecting early stages of infection. Virus particles were found in skin biopsy specimens as early as 12 days post inoculation. Immature naked particles appeared first in the nucleus; later particles were enveloped in the cytoplasm and enclosed in cytoplasmic inclusion bodies. No evidence for continued virus replication was seen in feather follicles after an initial burst of heavy virus production, which lasted several weeks. Residual virus, however, was found occasionally in cytoplasmic inclusion bodies within keratinized material near the feathers. This was believed to contribute to the long-term shedding of infectious virus into the environment.
采用三种评估系统，即免疫扩散、荧光抗体（FA）和电子显微镜（EM），来追踪接种鸡体内马立克氏病病毒的形态发生。在这三种系统中，EM和FA在检测感染早期阶段最为敏感。接种后12天，在皮肤活检标本中就发现了病毒颗粒。未成熟的裸病毒颗粒首先出现在细胞核中；随后病毒颗粒在细胞质中被包膜包裹，并被包在细胞质包涵体内。在最初几周大量产生病毒之后，未发现羽毛滤泡中有持续病毒复制的迹象。然而，在羽毛附近角质化物质内的细胞质包涵体中偶尔发现残留病毒。据信这有助于传染性病毒长期向环境中脱落。
