Bumann D, Oesterhelt D
Department of Membrane Biochemistry, Max Planck Institute for Biochemistry, Martinsried, Germany.
Biochemistry. 1994 Sep 13;33(36):10906-10. doi: 10.1021/bi00202a009.
Oxygen-evolving photosystem II complexes were isolated from the green alga Chlamydomonas reinhardtii by selective solubilization of thylakoid membranes with dodecyl maltoside followed by density gradient centrifugation and anion-exchange chromatography. In the presence of CaCl2 and K3[Fe(CN)6] the complexes evolved oxygen at rates exceeding 1000 mumol (mg of chl)-1 h-1. The particles contained 40 chlorophylls a and had properties very similar to those of PSII isolated from higher plants. Chlamydomonas reinhardtii is now the first organism which can be used for both site-directed mutagenesis and detailed biochemical and biophysical characterization of oxygen-evolving photosystem II. It seems therefore to be an ideal model organism for investigation of structure-function relationships in photosynthetic oxygen evolution.
通过用十二烷基麦芽糖苷选择性溶解类囊体膜，然后进行密度梯度离心和阴离子交换色谱法，从莱茵衣藻绿藻中分离出了放氧光系统II复合物。在氯化钙和铁氰化钾存在的情况下，这些复合物以超过1000 μmol（mg叶绿素）-1 h-1的速率释放氧气。这些颗粒含有40个叶绿素a，其性质与从高等植物中分离出的PSII非常相似。莱茵衣藻现在是第一种可用于定点诱变以及对放氧光系统II进行详细生化和生物物理表征的生物。因此，它似乎是研究光合放氧中结构-功能关系的理想模式生物。
