Bumann D, Oesterhelt D
Department of Membrane Biochemistry, Max Planck Institute for Biochemistry, Martinsried, Germany.
Proc Natl Acad Sci U S A. 1995 Dec 19;92(26):12195-9. doi: 10.1073/pnas.92.26.12195.
Pigments destroyed during photoinhibition of water-splitting photosystem II core complexes from the green alga Chlamydomonas reinhardtii were studied. Under conditions of a transiently inactivated donor side, illumination leads to an irreversible inhibition of the electron transfer at the donor side that is paralleled by the destruction of chlorophylls a absorbing maximally around 674 and 682 nm. The observed stochiometry of 1 +/- 0.1 destroyed chlorophyll per inhibited photosystem II suggests that chlorophyll destruction could be the primary photodamage causing the inhibition of photosystem II under these conditions.
对莱茵衣藻水裂解光系统II核心复合物光抑制过程中被破坏的色素进行了研究。在供体侧瞬时失活的条件下,光照会导致供体侧电子传递的不可逆抑制,与此同时,在674和682nm左右吸收峰最大的叶绿素a被破坏。观察到每个受抑制的光系统II有1±0.1个被破坏的叶绿素的化学计量关系,这表明在这些条件下,叶绿素破坏可能是导致光系统II抑制的主要光损伤。