Nilsson D, Johansen E
Department of Genetics, Chr. Hansen's Laboratorium, Hørsholm, Denmark.
Biochim Biophys Acta. 1994 Sep 13;1219(1):141-4. doi: 10.1016/0167-4781(94)90256-9.
A tRNA operon (trnA) from Lactococcus lactis consisting of seven tRNA genes and a 5S rRNA gene was cloned and sequenced. Promoter-fusion of the trnA promoter to a promoter-less beta-galactosidase gene of Leuconostoc mesenteroides resulted in high levels of beta-galactosidase activity in L. lactis. Searching for sequences with similarity to the sequence of the promoter region revealed a consensus sequence of promoters preceeding rRNA operons and tRNA operons from Lactococcus species including a not previously described conserved sequence (AGTT).
克隆并测序了来自乳酸乳球菌的一个tRNA操纵子（trnA），它由7个tRNA基因和一个5S rRNA基因组成。将trnA启动子与肠系膜明串珠菌无启动子的β-半乳糖苷酶基因进行启动子融合，导致乳酸乳球菌中β-半乳糖苷酶活性水平较高。搜索与启动子区域序列相似的序列，发现了乳酸乳球菌属rRNA操纵子和tRNA操纵子之前的启动子共有序列，包括一个以前未描述的保守序列（AGTT）。
