• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

嗜热古细菌嗜热甲烷杆菌中编码5S核糖体RNA和转运RNA的基因。

Genes encoding 5S rRNA and tRNAs in the extremely thermophilic archaebacterium Methanothermus fervidus.

作者信息

Haas E S, Daniels C J, Reeve J N

机构信息

Molecular, Cellular and Developmental Biology Program, Ohio State University, Columbus 43210.

出版信息

Gene. 1989 Apr 30;77(2):253-63. doi: 10.1016/0378-1119(89)90073-5.

DOI:10.1016/0378-1119(89)90073-5
PMID:2473943
Abstract

Methanothermus fervidus was shown to have two 5S rRNA-encoding genes linked in rRNA operons to 16S and 23S rRNA-encoding genes. Sequencing of a cloned 5S rRNA gene confirmed that M. fervidus is a member of the Methanobacteriales, although its 5S rRNA is also similar in both primary sequence and predicted secondary structure to the 5S rRNA of the non-methanogenic, but also extremely thermophilic archaebacterium, Thermococcus celer. Two clusters of tRNA genes have also been cloned and sequenced form M. fervidus. The smaller cluster, cloned in pET5401, is composed of 5'-tRNA(UGUThr)-tRNA(UGGPro)-tRNA(GUCAsp)-tRNA(UUUL ys)-3' and the larger cluster, cloned in pET5475, is composed of 5'-tRNA(GUUAsn)-tRNA(CAUMet)-tRNA(UUCGlu)-tRNA(UAGL eu)-tRNA(GUGHis)-3'. The encoded tRNAs, with the exception of the tRNA(Leu), translate abundant codons in M. fervidus. The tRNA genes do not contain introns or encode 3'-terminal CCA residues. Homologous clusters of tRNA genes have been sequenced from Methanococcus vannielii and Methanococcus voltae, so that comparisons of transcription signals, gene organizations and primary sequences can be made and features possibly related to thermostability identified. During evolution, a 5S rRNA gene appears to have been incorporated into the cluster of tRNA genes in the methanococci but not in M. fervidus.

摘要

嗜热甲烷栖热菌被证明有两个编码5S rRNA的基因，它们在rRNA操纵子中与编码16S和23S rRNA的基因相连。对一个克隆的5S rRNA基因进行测序证实，嗜热甲烷栖热菌是甲烷杆菌目的成员，尽管其5S rRNA在一级序列和预测的二级结构上也与非产甲烷但同样嗜热的古细菌嗜热栖热球菌的5S rRNA相似。也已从嗜热甲烷栖热菌中克隆并测序了两个tRNA基因簇。较小的基因簇克隆于pET5401，由5'-tRNA(UGUThr)-tRNA(UGGPro)-tRNA(GUCAsp)-tRNA(UUULys)-3'组成，而较大的基因簇克隆于pET5475，由5'-tRNA(GUUAsn)-tRNA(CAUMet)-tRNA(UUCGlu)-tRNA(UAGLeu)-tRNA(GUGHis)-3'组成。除了tRNA(Leu)外，所编码的tRNA可翻译嗜热甲烷栖热菌中丰富的密码子。这些tRNA基因不含内含子，也不编码3'-末端CCA残基。已对万氏甲烷球菌和沃氏甲烷球菌的tRNA基因同源簇进行了测序，从而可以对转录信号、基因组织和一级序列进行比较，并确定可能与热稳定性相关的特征。在进化过程中，一个5S rRNA基因似乎已整合到甲烷球菌的tRNA基因簇中，但在嗜热甲烷栖热菌中却没有。

相似文献

1
Genes encoding 5S rRNA and tRNAs in the extremely thermophilic archaebacterium Methanothermus fervidus.嗜热古细菌嗜热甲烷杆菌中编码5S核糖体RNA和转运RNA的基因。
Gene. 1989 Apr 30;77(2):253-63. doi: 10.1016/0378-1119(89)90073-5.
2
Genes encoding the 7S RNA and tRNA(Ser) are linked to one of the two rRNA operons in the genome of the extremely thermophilic archaebacterium Methanothermus fervidus.编码7S RNA和tRNA(Ser)的基因与嗜热古细菌嗜热栖热菌基因组中的两个rRNA操纵子之一相连。
Gene. 1990 May 31;90(1):51-9. doi: 10.1016/0378-1119(90)90438-w.
3
Apparent operon for a 5S ribosomal RNA gene and for tRNA genes in the archaebacterium Methanococcus vannielii.嗜甲烷球菌（Methanococcus vannielii）中5S核糖体RNA基因和tRNA基因的明显操纵子。
Mol Gen Genet. 1984;196(1):146-51. doi: 10.1007/BF00334107.
4
Cloning and characterization of a cluster of genes coding for 5S rRNA and three tRNAs from Azospirillum lipoferum.脂环酸芽孢杆菌中编码5S rRNA和三种tRNA的一组基因的克隆与特性分析。
Biochem Mol Biol Int. 1994 Jul;33(4):793-805.
5
Structure and comparative analysis of the genes encoding component C of methyl coenzyme M reductase in the extremely thermophilic archaebacterium Methanothermus fervidus.嗜热栖热甲烷菌中甲基辅酶M还原酶C组分编码基因的结构及比较分析
J Bacteriol. 1988 Oct;170(10):4718-26. doi: 10.1128/jb.170.10.4718-4726.1988.
6
Transcription in vitro and in vivo of the 7S RNA gene associated with the ribosomal RNA operon in the hyperthermophilic archaeon Methanothermus fervidus.嗜热古菌嗜热栖热甲烷菌中与核糖体RNA操纵子相关的7S RNA基因的体外和体内转录。
FEMS Microbiol Lett. 1992 Nov 1;77(1-3):95-101. doi: 10.1016/0378-1097(92)90138-e.
7
Organization of ribosomal RNA genes from a Loofah witches' broom phytoplasma.来自丝瓜疯长病植原体的核糖体RNA基因的组织
DNA Cell Biol. 2001 Feb;20(2):115-22. doi: 10.1089/104454901750070328.
8
Sequence of 5S ribosomal RNA gene regions and their products in the archaebacterium Halobacterium volcanii.嗜盐古菌沃氏嗜盐菌中5S核糖体RNA基因区域及其产物的序列
Mol Gen Genet. 1985;198(2):270-4. doi: 10.1007/BF00383005.
9
DNA sequence of the 16S rRNA/23S rRNA intercistronic spacer of two rDNA operons of the archaebacterium Methanococcus vannielii.嗜甲烷球菌两个rDNA操纵子的16S rRNA/23S rRNA基因间间隔区的DNA序列。
Nucleic Acids Res. 1983 Nov 11;11(21):7537-44. doi: 10.1093/nar/11.21.7537.
10
Sequence of a hexameric tRNA gene cluster associated with rRNA genes in Lactobacillus bulgaricus.与保加利亚乳杆菌核糖体RNA基因相关的六聚体转运RNA基因簇序列
Nucleic Acids Res. 1989 Jun 26;17(12):4873.

引用本文的文献

1
Transfer RNA Modification Enzymes from Thermophiles and Their Modified Nucleosides in tRNA.嗜热菌的转运RNA修饰酶及其tRNA中的修饰核苷
Microorganisms. 2018 Oct 20;6(4):110. doi: 10.3390/microorganisms6040110.
2
RNA-Seq analyses reveal the order of tRNA processing events and the maturation of C/D box and CRISPR RNAs in the hyperthermophile Methanopyrus kandleri.RNA-Seq 分析揭示了嗜热古菌 Methanopyrus kandleri 中 tRNA 加工事件的顺序以及 C/D 盒和 CRISPR RNA 的成熟过程。
Nucleic Acids Res. 2013 Jul;41(12):6250-8. doi: 10.1093/nar/gkt317. Epub 2013 Apr 25.
3
Transfer RNA gene organization and RNase P.
转运RNA基因组织与核糖核酸酶P
Mol Biol Rep. 1995;22(2-3):181-5. doi: 10.1007/BF00988726.
4
Cloning, functional organization, transcript studies, and phylogenetic analysis of the complete nitrogenase structural genes (nifHDK2) and associated genes in the archaeon Methanosarcina barkeri 227.巴氏甲烷八叠球菌227中完整固氮酶结构基因（nifHDK2）及相关基因的克隆、功能组织、转录研究和系统发育分析
J Bacteriol. 1996 Jan;178(1):143-8. doi: 10.1128/jb.178.1.143-148.1996.
5
Characterization and phylogeny of mcrII, a gene cluster encoding an isoenzyme of methyl coenzyme M reductase from hyperthermophilic Methanothermus fervidus.嗜热栖热甲烷菌中编码甲基辅酶M还原酶同工酶的基因簇mcrII的特性及系统发育分析
Mol Gen Genet. 1994 Apr;243(2):198-206. doi: 10.1007/BF00280317.
6
Sequence and structure comparison suggest that methionine aminopeptidase, prolidase, aminopeptidase P, and creatinase share a common fold.序列和结构比较表明，甲硫氨酸氨基肽酶、脯氨酰二肽酶、氨基肽酶P和肌酸酶具有共同的折叠结构。
Proc Natl Acad Sci U S A. 1994 Mar 29;91(7):2473-7. doi: 10.1073/pnas.91.7.2473.
7
New nucleotide sequence data on the EMBL File Server.欧洲分子生物学实验室文件服务器上的新核苷酸序列数据。
Nucleic Acids Res. 1989 Sep 25;17(18):7553-78. doi: 10.1093/nar/17.18.7553.
8
HMf, a DNA-binding protein isolated from the hyperthermophilic archaeon Methanothermus fervidus, is most closely related to histones.HMf是一种从嗜热古菌嗜热甲烷栖热菌中分离出来的DNA结合蛋白，与组蛋白关系最为密切。
Proc Natl Acad Sci U S A. 1990 Aug;87(15):5788-91. doi: 10.1073/pnas.87.15.5788.
9
Compilation of tRNA sequences and sequences of tRNA genes.tRNA序列及tRNA基因序列的汇编。
Nucleic Acids Res. 1991 Apr 25;19 Suppl(Suppl):2127-71. doi: 10.1093/nar/19.suppl.2127.