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人TATA结合蛋白CAG三联体重复序列在PCR扩增过程中的不稳定性

Instability of human TATA-binding protein CAG triplet repeats during amplification by PCR.

作者信息

Holstege F C, van der Vliet P C, Timmers H T

机构信息

Laboratory for Physiological Chemistry, Utrecht University, The Netherlands.

出版信息

Biochim Biophys Acta. 1994 Sep 13;1219(1):157-9. doi: 10.1016/0167-4781(94)90260-7.

DOI:10.1016/0167-4781(94)90260-7
PMID:8086455
Abstract

Polymerase chain reaction (PCR) of a TATA-binding protein cDNA that contains CAG triplet repeats results in heterogeneous products. This is caused by a variable loss in the number of CAG triplets. Sequence analysis of PCR products suggests that instability increases with repeat length.

摘要

对含有CAG三联体重复序列的TATA结合蛋白cDNA进行聚合酶链反应(PCR)会产生异质产物。这是由CAG三联体数量的可变丢失引起的。PCR产物的序列分析表明,不稳定性随着重复长度的增加而增加。

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