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鼠伤寒沙门氏菌14028s编码RpoS(KatF)σ因子的基因的克隆与测序

Cloning and sequencing of the gene encoding the RpoS (KatF) sigma factor from Salmonella typhimurium 14028s.

作者信息

Prince R W, Xu Y, Libby S J, Fang F C

机构信息

Division of Infectious Diseases B-168, University of Colorado Health Sciences Center, Denver 80262.

出版信息

Biochim Biophys Acta. 1994 Sep 13;1219(1):198-200. doi: 10.1016/0167-4781(94)90271-2.

Abstract

The gene encoding the alternative sigma factor RpoS in Salmonella typhimurium was cloned by its ability to complement acid susceptibility in rpoS mutant Escherichia coli. Sequence determination and comparison with rpoS from E. coli demonstrates a high degree of conservation, although significant differences are found within the extragenic regulatory regions.

摘要

通过其在rpoS突变型大肠杆菌中补偿酸敏感性的能力,克隆了鼠伤寒沙门氏菌中编码替代σ因子RpoS的基因。序列测定以及与大肠杆菌rpoS的比较表明,尽管在外基因调控区域内发现了显著差异,但仍具有高度保守性。

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