Hofstad T
Department of Microbiology and Immunology, Gade Institute, University of Bergen, Norway.
Clin Infect Dis. 1994 May;18 Suppl 4:S250-2. doi: 10.1093/clinids/18.supplement_4.s250.
Results of genetic and biochemical analyses have broadened our understanding of taxonomic relationships among groups of anaerobic bacteria and have led to a better understanding of the pathogenesis of infection. Conventional bacteriologic methods are still of prime importance for the detection and identification of anaerobic pathogens. The use of nucleic acid probes has so far been restricted to research laboratories. A polymerase chain reaction-generated probe would be most useful for the rapid detection of toxigenic Clostridium difficile in feces. Probes are needed for detection of periodontopathogenic bacteria in dental plaque. Use of nucleic acid probes may become a useful adjunct to classic methodology in reference and teaching laboratories.
基因和生化分析结果拓宽了我们对厌氧细菌菌群间分类关系的理解，并使我们对感染的发病机制有了更好的认识。传统细菌学方法对于厌氧病原体的检测和鉴定仍然至关重要。迄今为止，核酸探针的使用仅限于研究实验室。聚合酶链反应生成的探针对于粪便中产毒艰难梭菌的快速检测最为有用。检测牙菌斑中牙周致病细菌需要探针。在参考实验室和教学实验室中，核酸探针的使用可能会成为经典方法的有用辅助手段。
