Wang J, Li L, Li S, Cui H, Shen G
Cancer Institute, CAMS & PUMC, Beijing.
Chin Med Sci J. 1994 Mar;9(1):24-8.
Expression of c-myc oncogene transcripts in colorectal neoplasia was studied in paraffin embedded tissue sections from 25 patients undergoing surgery and from the rectal carcinoma cell line HR-8348 by using in situ hybridization, and its amplification was investigated in tumor and normal mucosa tissue from 25 coloproctomy samples by slot blot hybridization. Overexpression of this gene was seen in 78% (7/9) of the benign adenomas and 91% (20/22) of the malignancies sampled. There was no significant correlation between overexpression and the histologic type or grade, and no significant relationship between the level of expression and clinical stage was found, although overexpression was apparently more common in tumors with metastasis. Amplification of the gene was found in 0 of 4 benign adenomas and 7 of 22 malignancies. No obvious correlation was found between amplification and histological type or grade, though amplification was more frequent in tumors with metastasis. Amplification was also found in 2 adenomas with malignant change. The results suggest that multiple factors are involved in the progression of colorectal cancer, and in situ hybridization with a nonradiolabeled probe is useful in the detection of gene expression.
采用原位杂交技术，对25例接受手术患者的石蜡包埋组织切片以及直肠癌细胞系HR - 8348中c - myc癌基因转录本的表达进行了研究，并通过狭缝印迹杂交法对25份结肠直肠切除标本的肿瘤组织和正常黏膜组织中该基因的扩增情况进行了检测。在所取样本中，78%（7/9）的良性腺瘤和91%（20/22）的恶性肿瘤出现了该基因的过表达。过表达与组织学类型或分级之间无显著相关性，且未发现表达水平与临床分期之间存在显著关系，不过过表达在有转移的肿瘤中显然更为常见。在4例良性腺瘤中未发现该基因扩增，而在22例恶性肿瘤中有7例出现扩增。扩增与组织学类型或分级之间未发现明显相关性，尽管在有转移的肿瘤中扩增更为频繁。在2例发生恶变的腺瘤中也发现了扩增。结果表明，结直肠癌的进展涉及多种因素，并且使用非放射性标记探针进行原位杂交有助于检测基因表达。
