Smith D R, Myint T, Goh H S
Department of Colorectal Surgery, Singapore General Hospital.
Br J Cancer. 1993 Aug;68(2):407-13. doi: 10.1038/bjc.1993.350.
Alterations in the c-myc proto-oncogene in colorectal cancer were studied at the level of RNA expression, gene amplification and rearrangements. One hundred cases of colorectal cancer, stratified by Dukes' stage were examined. The level of messenger RNA expression was measured in tumours and matched normal mucosa from the same patient. Between 5 and 400 fold over-expression was found in 66% of tumours. Neither the presence nor the level of over-expression correlated with tumour staging. A significant correlation (P < 0.01) was found between over-expression of c-myc in tumours and the presence of synchronous adenomas elsewhere in the colon. In contrast to other tumours, no rearrangements of the gene were found on Southern analysis of colorectal cancers. Similarly, amplification of the gene was not found in the cancers examined.
在RNA表达、基因扩增和重排水平上研究了结直肠癌中c-myc原癌基因的改变。对100例按杜克分期分层的结直肠癌病例进行了检查。测量了肿瘤及同一患者匹配的正常黏膜中信使RNA的表达水平。在66%的肿瘤中发现了5至400倍的过表达。过表达的存在与否及水平均与肿瘤分期无关。肿瘤中c-myc的过表达与结肠其他部位同步腺瘤的存在之间存在显著相关性（P<0.01）。与其他肿瘤不同，在结直肠癌的Southern分析中未发现该基因的重排。同样，在所检查的癌症中未发现该基因的扩增。
