Bourges M, Vanneuville G
Département de Microscopie électronique, Faculté de Médecine, Université d'Auvergene, Clermont Ferrand, France.
Bull Assoc Anat (Nancy). 1994 Jun;78(241):5-8.
Preliminary histological observations concerning interspinal ligament, at level T1 T2 and far the 14th, 24th and 37th post-ovulatory (P.O.) weeks, are presented here. In an histological, then biological context, these observation take place into an evolutive study of the muscular and ligamentous material of the human fetus spine. The paraffin included biopsy sections were examined after being colored with Mallory blue and picro-indigo-carmin chlorhydric orcein. After 14 weeks P.O., an heterogeneous structure appears with muscular, mixt and fibrous fascicules in a polygonal cellulo-fibrous network. After 24 weeks P.O., the structure is entirely fibrous, in a long, plexiform web. The evolution become more acute after 37 weeks P.O. After chlorhydric orcein coloration, no elastic fibers were observed in the collagen network, during the first two stages of observation cholorhydric orcein coloration, however, revealed a small quantity of them, 37 weeks after ovulation.
本文呈现了关于T1-T2水平以及排卵后（P.O.）第14、24和37周的棘间韧带的初步组织学观察结果。在组织学以及生物学背景下，这些观察是在对人类胎儿脊柱肌肉和韧带材料的进化研究中进行的。对石蜡包埋的活检切片用马洛里蓝和苦味酸靛胭脂盐酸orcein染色后进行检查。排卵后14周后，在多边形细胞纤维网络中出现了由肌肉、混合和纤维束组成的异质结构。排卵后24周，结构完全为纤维状，呈长的、网状。排卵后37周进化变得更加明显。在用盐酸orcein染色后，在观察的前两个阶段，胶原网络中未观察到弹性纤维，然而，排卵37周后发现了少量弹性纤维。
