Kvasnikov E I, Aĭzenman B E, Solomko E F, Kiprianova E A, Boĭko O I
Mikrobiologiia. 1975 Jan-Feb;44(1):55-60.
The ability to assimilate n-alkanes form hexane to decane was studied among 495 collection strains and 27 freshly isolated strains belonging to the genus Pseudomonas. All freshly isolated strains and over one third of collection cultures of Ps. aurantiaca grow on mineral media with n-alkanes of low molecular weight, but do not assimilate heavy paraffins. The strains of Ps. aeruginosa, Ps. fluorescens and Ps. putida, isolated from oilbearing soils, and individual collection cultures, belonging to the two latter species, can assimilate both n-alkanes of low molecular weight (C6--C10) and heavy paraffins. Contrary to Ps. aurantiaca, other species of the Pseudomonas genus lose the ability to assimilate n-alkanes of low molecular weight after cultivation on rich organic media. An increase in the concentration of the mixture of low molecular weight paraffins (to 20 per cent by volume) has no toxic effect on the Pseudomonas bacteria whose biomass has a high content of protein and all necessary amino acids. The strains of Ps. aurantiaca produce a highly active antibiotic preparation consisting of floroglucine derivatives on the defined medium with n-alkanes of low molecular weight. The ratio between components of the preparation obtained on the media with n-alkanes and on the optimal organic media is different.
在495株保藏菌株和27株新分离的假单胞菌属菌株中,研究了同化从己烷到癸烷的正构烷烃的能力。所有新分离的菌株以及超过三分之一的橙色假单胞菌保藏培养物都能在含有低分子量正构烷烃的矿物培养基上生长,但不能同化重质石蜡。从含油土壤中分离出的铜绿假单胞菌、荧光假单胞菌和恶臭假单胞菌菌株,以及属于后两个种的个别保藏培养物,既能同化低分子量的正构烷烃(C6 - C10),也能同化重质石蜡。与橙色假单胞菌相反,假单胞菌属的其他种在富含有机培养基上培养后失去了同化低分子量正构烷烃的能力。低分子量石蜡混合物浓度的增加(至体积的20%)对生物量中蛋白质和所有必需氨基酸含量高的假单胞菌没有毒性作用。橙色假单胞菌菌株在含有低分子量正构烷烃的限定培养基上产生一种由间苯三酚衍生物组成的高活性抗生素制剂。在含有正构烷烃的培养基上和最佳有机培养基上获得的制剂成分之间的比例不同。