Illarionov E F, Samoĭlov P M, Eroshin V K
Mikrobiologiia. 1977 Nov-Dec;46(6):1027-33.
Protein disc-electrophoregrams of cell-free homogenates have been compared in the "non-adapted" and "preliminarily-adapted" to n-hexane strains of Pseudomonas aeruginosa. A statistically reliable increase in the content of protein by 60 per cent was found in fractions a (M. W. 70,000) and b (M. W. 130,000) when the cells of both strains were gorwn on hexane as compared to the cells cultivated on glucose. The content of protein in each a and b band was about 4 per cent of the total cell protein before the induction by hexane. No differences were found when the cells of the two strains were grown on identical substrates. The induced proteins are supposed to belong to the system of primary oxidation of n-alkanes with short chains and to be localized in the cytoplasm of Ps. aeruginosa.
对铜绿假单胞菌“未适应”和“初步适应”正己烷的菌株的无细胞匀浆的蛋白质圆盘电泳图进行了比较。当两种菌株的细胞在己烷上生长时,与在葡萄糖上培养的细胞相比,发现组分a(分子量70,000)和组分b(分子量130,000)中的蛋白质含量有统计学上可靠的60%的增加。在己烷诱导之前,每个a带和b带中的蛋白质含量约占总细胞蛋白质的4%。当两种菌株的细胞在相同底物上生长时,未发现差异。推测诱导产生的蛋白质属于短链正构烷烃初级氧化系统,定位于铜绿假单胞菌的细胞质中。