Jung K, Kühler S, Klotzek S, Becker S, Henke W
Department of Urology, University Hospital Charité, Humboldt University Berlin, FRG.
Enzyme Protein. 1993;47(3):149-55. doi: 10.1159/000468670.
The behavior of the catalytic activities of the enzymes superoxide dismutase, catalase, glutathione peroxidase, glutathione S-transferase and glutathione reductase was tested in rat liver and kidney homogenates stored at 4, -20 and -70 degrees C and in corresponding tissue samples stored at -70 degrees C. The stabilities of enzymes were different for various enzymes and were dependent on the organ (liver, kidney) and the storage temperature. The storage temperature of -70 degrees C guaranteed the best stability and the five enzymes investigated were sufficiently stable in preserved tissue samples or in homogenates prepared with conventional mannit/sucrose homogenization solution. Under such conditions, these enzymes were stable over at least 4 or 1 weeks, respectively.
在4℃、-20℃和-70℃储存的大鼠肝脏和肾脏匀浆以及在-70℃储存的相应组织样本中，测试了超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶、谷胱甘肽S-转移酶和谷胱甘肽还原酶的催化活性行为。不同酶的稳定性不同，并且取决于器官（肝脏、肾脏）和储存温度。-70℃的储存温度保证了最佳稳定性，所研究的五种酶在保存的组织样本或用传统甘露醇/蔗糖匀浆溶液制备的匀浆中足够稳定。在这种条件下，这些酶分别至少稳定4周或1周。
