Lecellier G, Silar P
Centre de Génétique Moléculaire du C.N.R.S., Gif sur Yvette, France.
Curr Genet. 1994 Feb;25(2):122-3. doi: 10.1007/BF00309536.
We have developed rapid and economic methods for the isolation of nucleic acids from filamentous fungi. The main advantages of these methods are: (1) the mycelium is directly recovered from a Petri-dish culture, (2) the complete experiment takes place in microfuge tubes, (3) it is very fast and allows for the processing of 24 samples in the same day, and (4) up to 100 micrograms of total DNA or RNA are recovered, both of which are sufficiently pure for most purposes. Of particular interest is the recovery of large amounts of mitochondrial DNA as visualised by electrophoresis in ethidium bromide-stained gels.
我们已经开发出了从丝状真菌中分离核酸的快速且经济的方法。这些方法的主要优点包括：（1）菌丝体直接从培养皿培养物中回收；（2）整个实验在微量离心管中进行；（3）速度非常快，一天内可处理24个样本；（4）可回收多达100微克的总DNA或RNA，二者纯度足以满足大多数用途。特别值得一提的是，通过溴化乙锭染色凝胶电泳可视化可回收大量线粒体DNA。
