Agrewala J N, Vinay D S, Joshi A, Mishra G C
Institute of Microbial Technology, Chandigarh, India.
Eur J Immunol. 1994 Sep;24(9):2092-7. doi: 10.1002/eji.1830240924.
In the present study, we describe the potential co-stimulatory role of a macrophage membrane-associated protein of 150 kDa (M150). The protein was isolated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and was found to be a single molecule on two-dimensional gel electrophoresis. The molecule was re-constituted in phosphatidyl choline vesicles and tested for its ability to promote the proliferation and the secretion of lymphokines from T helper (Th) cells. The reconstituted M150 induced a significant proliferation of anti-CD3 monoclonal antibody (mAb)-stimulated ovalbumin-specific CD4+ T cells. Further, Th cells activated with this molecule in the presence of anti-CD3 mAb mainly secreted interleukin (IL)-2 and interferon-gamma but not IL-4. M150 could not promote the proliferation of Th cells, or lymphokine secretion in the absence of anti-CD3 mAb. These observations suggest that M150 acts by selectively activating a Th1-like immune response.
在本研究中，我们描述了一种150 kDa巨噬细胞膜相关蛋白（M150）的潜在共刺激作用。该蛋白通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分离，在二维凝胶电泳中被发现是单一分子。该分子在磷脂酰胆碱囊泡中重构，并测试其促进T辅助（Th）细胞增殖和分泌淋巴因子的能力。重构的M150诱导抗CD3单克隆抗体（mAb）刺激的卵清蛋白特异性CD4 + T细胞显著增殖。此外，在抗CD3 mAb存在下用该分子激活的Th细胞主要分泌白细胞介素（IL）-2和干扰素-γ，而不分泌IL-4。在没有抗CD3 mAb的情况下，M150不能促进Th细胞增殖或淋巴因子分泌。这些观察结果表明，M150通过选择性激活类似Th1的免疫反应发挥作用。
