Lee K L, Rega M E, Watson R R, Campbell C C
Sabouraudia. 1975 Jul;13(2):132-41. doi: 10.1080/00362177585190251.
Specificity of aminopeptidase(s) was fluorimetrically determined in the yeast phase of Histoplasma capsulatum, H. duboisii, H. farciminosum, Blastomyces dermatitidis, Paracoccidioides brasiliensis, Candida albicans and Crytococcus neoformans. After individually incubating each of 26 amino acid-beta-naphthylamides with each yeast, the amount of each amino acid-beta-naphthylamide hydrolyzed was determined by measuring the free naphythylamine. This resulted in a reproducible profile of the aminopeptidase(s) for each fungus when medium, growth time, size of inoculum and incubation period were standardized. This technique provided a rapid and specific means of identification and differentiation among these yeasts. Specific amino acids were identified from the profile of each yeast as those rapidly liberated by the aminopeptidase(s) of that yeast. These amino acids delineated the amino acid requirement for the normal growth of each yeast.
采用荧光法测定荚膜组织胞浆菌、杜波伊斯组织胞浆菌、鼻疽组织胞浆菌、皮炎芽生菌、巴西副球孢子菌、白色念珠菌和新型隐球菌酵母相中的氨肽酶特异性。将26种氨基酸-β-萘酰胺分别与每种酵母单独孵育后，通过测量游离萘胺来确定每种氨基酸-β-萘酰胺的水解量。当培养基、生长时间、接种物大小和孵育期标准化后，可得到每种真菌氨肽酶的可重复图谱。该技术为这些酵母的鉴定和区分提供了一种快速且特异的方法。从每种酵母的图谱中鉴定出特定氨基酸，即该酵母氨肽酶能快速释放的氨基酸。这些氨基酸描绘了每种酵母正常生长所需的氨基酸。
