Labastie M C, Bories D, Chabret C, Grégoire J M, Chrétien S, Roméo P H
INSERM U.91, Hôpital Henri Mondor, Créteil, France.
Genomics. 1994 May 1;21(1):1-6. doi: 10.1006/geno.1994.1217.
GATA3, a member of the GATA family that is abundantly expressed in the T-lymphocyte lineage, is thought to participate in T-cell receptor gene activation through binding to enhancers. To understand GATA3 gene regulation, we cloned the human gene and the 5' end of the mouse GATA3 gene. We show that the human GATA3 gene contains six exons distributed over 17 kb of DNA. The two human GATA3 zinc fingers are encoded by two separate exons highly conserved with those of GATA1, but no other structural homologies between these two genes can be found. The human and mouse GATA3 transcription units start at a major initiation site. The promoter sequence analysis of these two genes revealed that they are embedded within a CpG island and share structural features often found in the promoters of housekeeping genes. Finally, we show that a DNA fragment containing the human GATA3 transcription unit, 3 kb upstream from the initiation site and 4 kb downstream from the polyadenylation site, displays T-cell specificity.
GATA3是GATA家族的成员之一，在T淋巴细胞谱系中大量表达，被认为通过与增强子结合参与T细胞受体基因的激活。为了了解GATA3基因的调控机制，我们克隆了人类基因和小鼠GATA3基因的5'端。我们发现人类GATA3基因包含六个外显子，分布在17 kb的DNA上。人类GATA3的两个锌指由两个独立的外显子编码，与GATA1的外显子高度保守，但这两个基因之间没有其他结构同源性。人类和小鼠的GATA3转录单元都从一个主要起始位点开始。对这两个基因的启动子序列分析表明，它们位于一个CpG岛内，具有管家基因启动子中常见的结构特征。最后，我们发现一个包含人类GATA3转录单元的DNA片段，在起始位点上游3 kb和多聚腺苷酸化位点下游4 kb处，表现出T细胞特异性。
