在睾丸细胞中表达的三个新的小鼠基因的染色体定位。

Chromosomal assignment of three novel mouse genes expressed in testicular cells.

作者信息

Lemaire L, Johnson K R, Bammer S, Petry P, Ruddle F H, Heinlein U A

机构信息

Institut für Genetik, Heinrich-Heine-Universität, Düsseldorf, Federal Republic of Germany.

出版信息

Genomics. 1994 May 15;21(2):409-14. doi: 10.1006/geno.1994.1284.

DOI:10.1006/geno.1994.1284

PMID:8088835

Abstract

The chromosomal positions of three genes that are selectively expressed in mouse testis cells have been identified. These genes include (i) TAZ83, which codes for an early- to mid-pachytene germ cell stage-expressed, cysteine-rich transmembrane protein (cyritestin) with homologies to various snake toxins and guinea pig sperm-egg fusion proteins; (ii) TNZ1, which is expressed in neonatal Leydig cells; and (iii) TAZ4, a testis-specific gene isolated by immunoscreening with antiserum raised against Sertoli cell membranes. Our experimental data, derived from chromosomal in situ hybridizations and RFLP studies of genetic backcrosses, indicate that (i) the TAZ83 (cyritestin) gene maps to chromosome 8, band A2, near the Plat locus; (ii) TNZ1 is located in the proximal region of chromosome 11; and (iii) TAZ4 is located at band D in the distal portion of chromosome 11, near the Hlr1 locus, with a related sequence, TAZ4-rs1, in the proximal part of chromosome 1.

摘要

已确定在小鼠睾丸细胞中选择性表达的三个基因的染色体位置。这些基因包括：（i）TAZ83，编码一种在粗线期早期至中期生殖细胞阶段表达的富含半胱氨酸的跨膜蛋白（cyritestin），与多种蛇毒素和豚鼠精卵融合蛋白具有同源性；（ii）TNZ1，在新生睾丸间质细胞中表达；（iii）TAZ4，一个通过用抗支持细胞膜抗血清进行免疫筛选分离出的睾丸特异性基因。我们从染色体原位杂交和遗传回交的RFLP研究中获得的实验数据表明：（i）TAZ83（cyritestin）基因定位于8号染色体A2带，靠近Plat基因座；（ii）TNZ1位于11号染色体的近端区域；（iii）TAZ4位于11号染色体远端的D带，靠近Hlr1基因座，在1号染色体近端有一个相关序列TAZ4-rs1。