Ohuchi J, Arai T, Kon Y, Asano A, Yamauchi H, Watanabe T
Laboratory of Experimental Animal Science, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.
Mol Reprod Dev. 2001 Aug;59(4):350-8. doi: 10.1002/mrd.1041.
During mammalian spermatogenesis, many specific molecules show the dynamics of expression and elimination, corresponding with the morphological differentiation of germ cells. We have isolated a novel cDNA designated F77 from mouse testis by cDNA subtractive hybridization between normal and sterile mice, using the C57BL/6 congenic strain for the hybrid sterilityhyphen;3 lpar;Hsthyphen;3rpar; allele from Mus spretus. The full-length F77 mRNA was 3.4 kb and showed significant nonmatching with entries in the databases. F77 was mapped at a proximal position between D8Mit212 and D8Mit138 on mouse chromosome 8, in which no corresponding genes related to its nucleotide sequence were found. F77 mRNA was not detected in any other organs except the testis of adult fertile mice. F77 protein was only seen in normal adult testis and epididymis. In contrast to normal C57BL/6 mice, F77 mRNA and protein were not seen in germ cell-deficient Kit(W)/Kit(Wv) mice. By in situ hybridization, F77 mRNA was detected mainly at round spermatids in the sexually mature testis, and immunohistochemical analysis revealed that F77 protein was located at the tail of elongated spermatids. We are proposing the name, sperm-tail-associated protein (Stap), for the gene encoding F77 cDNA. Mol. Reprod. Dev. 59: 350-358, 2001.
在哺乳动物精子发生过程中，许多特定分子呈现出表达和消除的动态变化，这与生殖细胞的形态分化相对应。我们利用来自小家鼠的C57BL/6同源品系的杂种不育-3（Hst-3）等位基因，通过正常小鼠和不育小鼠之间的cDNA消减杂交，从小鼠睾丸中分离出一个名为F77的新cDNA。F77全长mRNA为3.4 kb，与数据库中的条目显示出显著不匹配。F77被定位在小鼠8号染色体上D8Mit212和D8Mit138之间的近端位置，在该位置未发现与其核苷酸序列相关的对应基因。除成年可育小鼠的睾丸外，在任何其他器官中均未检测到F77 mRNA。F77蛋白仅在正常成年睾丸和附睾中可见。与正常C57BL/6小鼠相比，在生殖细胞缺陷的Kit(W)/Kit(Wv)小鼠中未观察到F77 mRNA和蛋白。通过原位杂交，在性成熟睾丸的圆形精子细胞中主要检测到F77 mRNA，免疫组织化学分析显示F77蛋白位于延长型精子细胞的尾部。我们提议将编码F77 cDNA的基因命名为精子尾部相关蛋白（Stap）。《分子生殖与发育》59: 350 - 358, 2001年。
