Owhashi M, Tomiyoshi F, Hayashi H
Faculty of Integrated Arts and Sciences, University of Tokushima, Japan.
Immunol Cell Biol. 1994 Jun;72(3):249-55. doi: 10.1038/icb.1994.37.
A neutrophil chemotactic factor (TfNCF) was isolated from the crude extract of Tritrichomonas foetus organisms by a combination of anion-exchange chromatography on DE52 and gel filtration on Sephacryl S200. TfNCF showed homogenicity by both PAGE and SDS-PAGE. The molecular weight of TfNCF was estimated to be 22 and 24 kDa, by Sephacryl S200 gel chromatography and by SDS-PAGE under reducing conditions. Immunization of TfNCF caused almost complete protection against T. foetus infection in mice. Western blot analysis probed with anti-TfNCF antibody showed that the epitopes on TfNCF were not commonly shared on the other components of T. foetus organisms nor other helminthous parasite-derived components. Furthermore, pre-incubation of neutrophils with antigens of other helminthous parasites or N-formylmethionyl-leucyl-phenylalanine did not affect the neutrophil chemotactic activity for TfNCF. These results suggest that TfNCF is a novel NCF consisting of unique epitopes for both antigenicity and neutrophil chemotactic activity. The role of NCF in the initiation of the immune response is discussed.
通过在DE52上进行阴离子交换色谱和在Sephacryl S200上进行凝胶过滤相结合的方法，从胎儿三毛滴虫生物体的粗提物中分离出一种中性粒细胞趋化因子（TfNCF）。TfNCF通过PAGE和SDS-PAGE均显示出均一性。通过Sephacryl S200凝胶色谱法和在还原条件下的SDS-PAGE估计，TfNCF的分子量为22 kDa和24 kDa。用TfNCF免疫可使小鼠几乎完全免受胎儿三毛滴虫感染。用抗TfNCF抗体进行的蛋白质印迹分析表明，TfNCF上的表位在胎儿三毛滴虫生物体的其他成分或其他蠕虫寄生虫衍生成分中并不常见。此外，用其他蠕虫寄生虫的抗原或N-甲酰甲硫氨酰-亮氨酰-苯丙氨酸对中性粒细胞进行预孵育，并不影响中性粒细胞对TfNCF的趋化活性。这些结果表明，TfNCF是一种新型的NCF，其抗原性和中性粒细胞趋化活性均具有独特的表位。文中讨论了NCF在免疫反应启动中的作用。
