Nijman H W, Van der Burg S H, Vierboom M P, Houbiers J G, Kast W M, Melief C J
Department of Immunohaematology and Blood Bank, University Hospital, Leiden, The Netherlands.
Immunol Lett. 1994 May;40(2):171-8. doi: 10.1016/0165-2478(94)90189-9.
Cell lineage-specific cellular proteins, oncogenes from viral or cellular origin and tumor suppressor genes encode tumor-specific/associated antigens. Such antigens can elicit an major compatibility complex (MHC) class I-restricted cytotoxic T lymphocyte (CTL) response, either naturally in cancer patients or following appropriate immunostimulation (in vitro or in vivo). The reported immune responses in humans to the melanoma-associated MAGE gene products, GP100 and tyrosinase, all self-proteins, support the idea to use wild-type p53 products as targets for T cells. An important step towards this goal is identification of potential p53 CTL epitopes. We identified the wild-type p53 peptides with the highest affinity to the HLA-A0201 molecule using two assays: the previously described MHC peptide-binding assay and the peptide competition assay. We obtained CTL against four p53 peptides with a high affinity for the HLA-A0201 molecule. These findings are discussed next to a short review concerning the p53 literature.
细胞谱系特异性细胞蛋白、病毒或细胞来源的癌基因以及肿瘤抑制基因编码肿瘤特异性/相关抗原。此类抗原可引发主要组织相容性复合体（MHC）I类限制性细胞毒性T淋巴细胞（CTL）反应，无论是在癌症患者体内自然发生，还是在适当的免疫刺激后（体外或体内）。在人类中报道的针对黑色素瘤相关MAGE基因产物、GP100和酪氨酸酶（均为自身蛋白）的免疫反应，支持了将野生型p53产物用作T细胞靶点的想法。朝着这一目标迈出的重要一步是鉴定潜在的p53 CTL表位。我们使用两种检测方法鉴定了与HLA - A0201分子具有最高亲和力的野生型p53肽：先前描述的MHC肽结合检测和肽竞争检测。我们获得了针对四种对HLA - A0201分子具有高亲和力的p53肽的CTL。这些发现将在对p53文献进行简短回顾的背景下进行讨论。
