Kiryu J, Shahidi M, Mori M T, Ogura Y, Asrani S, Zeimer R
Applied Physics Laboratory, UIC Eye Center, University of Illinois College of Medicine, Chicago.
Invest Ophthalmol Vis Sci. 1994 Sep;35(10):3724-31.
The choroidal microvasculature and its circulation are inadequately assessed by presently available techniques. Laser-targeted delivery was applied to generate local, repetitive angiograms of the choriocapillaris in primates.
Carboxyfluorescein was encapsulated in heat-sensitive liposomes and injected intravenously in monkeys. The liposome contents were then released locally in the choroid by application of a short heat pulse provided by an infrared laser. The bolus of dye spread rapidly downstream from the underlying arterioles into clusters of lobules. Video angiograms were generated with excitation illumination provided by an argon laser.
Laser-targeted delivery choroidal angiography performed on three monkeys indicated that the fluorescence was emitted mainly from the choriocapillaris. Clusters of irregular shape with well-defined margins were observed. Adjacent arteries typically supplied separate clusters that fit together like a jigsaw puzzle. The dynamic filling and emptying patterns, recorded at video rate, revealed that macular lobules were filled by a central arteriole and drained by a venous annulus. The average dye transit time through a lobule (n = 10) was 118 +/- 26 msec (mean +/- SD), and the dye transit velocity was 2.53 +/- 0.55 mm/sec.
This study clearly documents the segmental nature of the primate choroidal microvasculature. It also illustrates that choroidal angiography by laser-targeted dye delivery provides information useful for studying the response of the choriocapillaris to physiological and pathologic changes.
目前可用的技术对脉络膜微血管系统及其循环的评估并不充分。应用激光靶向递送技术来生成灵长类动物脉络膜毛细血管的局部重复血管造影图像。
将羧基荧光素包裹于热敏脂质体中，并经静脉注射到猴子体内。然后通过红外激光提供的短热脉冲使脂质体内容物在脉络膜局部释放。染料团从下层小动脉迅速向下游扩散到小叶簇中。利用氩激光提供的激发照明生成视频血管造影图像。
对三只猴子进行的激光靶向递送脉络膜血管造影显示，荧光主要发自脉络膜毛细血管。观察到边缘清晰的不规则形状的小叶簇。相邻动脉通常为分开的小叶簇供血，这些小叶簇组合在一起犹如拼图。以视频速率记录的动态充盈和排空模式显示，黄斑小叶由中央小动脉供血，并通过静脉环引流。染料通过一个小叶（n = 10）的平均通过时间为118 +/- 26毫秒（平均值 +/- 标准差），染料通过速度为2.53 +/- 0.55毫米/秒。
本研究清楚地证明了灵长类动物脉络膜微血管系统的节段性特征。它还表明，通过激光靶向染料递送进行脉络膜血管造影可为研究脉络膜毛细血管对生理和病理变化的反应提供有用信息。
