通过杂交捕获法检测人乳头瘤病毒及其可能的临床应用。
Human papillomavirus detection by hybrid capture and its possible clinical use.
作者信息
Farthing A, Masterson P, Mason W P, Vousden K H
机构信息
Ludwig Institute for Cancer Research, St Mary's Hospital, London.
出版信息
J Clin Pathol. 1994 Jul;47(7):649-52. doi: 10.1136/jcp.47.7.649.
Abstract
AIMS
To determine the sensitivity of the hybrid capture method for human papillomavirus (HPV) detection and potential clinical uses as a screening method for the identification of cervical intraepithelial neoplasia.
METHODS
The presence of oncogenic types of HPV was tested for in samples taken from the cervix at colposcopy, and compared with detection by polymerase chain reaction (PCR) in 60 patients. Both sets of results were corrected with the pathology determined by biopsy and smear cytology.
RESULTS
Hybrid capture detection showed 86% agreement with PCR. Eighty three percent of CIN 3 lesions, 62% of CIN 2, 59% of CIN 1 and 21% of normal controls were positive for oncogenic HPV types.
CONCLUSION
The hybrid capture detection method is reliable, sensitive, and easy to use. The addition of HPV testing to cytological screening would detect a greater proportion of cervical dysplasia with a higher false positive rate.
摘要
目的
确定杂交捕获法检测人乳头瘤病毒（HPV）的敏感性以及作为筛查方法用于识别宫颈上皮内瘤变的潜在临床用途。
方法
对60例患者在阴道镜检查时取自宫颈的样本检测致癌型HPV的存在情况，并与聚合酶链反应（PCR）检测结果进行比较。两组结果均根据活检和涂片细胞学确定的病理结果进行校正。
结果
杂交捕获检测与PCR检测结果的符合率为86%。83%的CIN 3病变、62%的CIN 2、59%的CIN 1以及21%的正常对照致癌型HPV检测呈阳性。
结论
杂交捕获检测方法可靠、灵敏且易于使用。在细胞学筛查中增加HPV检测能发现更大比例的宫颈发育异常，但假阳性率较高。
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