Yi Q, Lefvert A K
Immunologic Research Laboratory, Karolinska Hospital, Stockholm, Sweden.
J Immunol. 1994 Oct 1;153(7):3353-9.
Patients with myasthenia gravis have peripheral blood T cells that are stimulated to secrete IFN-gamma and IL-2 by human monoclonal anti-acetylcholine receptor and anti-idiotypic Abs. The human CD4+ Th cells may be subdivided into different subsets according to the pattern of cytokine production. To elucidate the subgroup of T cells involved in myasthenia gravis, we have analyzed IL-4-, IFN-gamma-, and IL-2-secretion patterns induced by two human disease-specific mAbs, one idiotypic and one anti-idiotypic Ab. T cells stimulated to secrete one or more of the cytokines were found in 33 of 34 patients. The idiotypic Ab stimulated IL-4 secretion in 19 of 34 patients, IFN-gamma secretion in 26 of 34 patients, and IL-2 secretion in 25 of 34. The anti-idiotypic Ab induced IL-4 secretion in 9 of 34 patients, IFN-gamma secretion in 29 of 34 patients, and IL-2 secretion in 28 of 34 patients. These T cell responses were MHC class II-restricted. According to the cytokine-secretion patterns, Id-reactive T cells might correspond to both Th1/Th2 and/or Th0 cells. The anti-idiotypic Ab more frequently induced a Th1-type response. This study indicates that different subsets of T lymphocytes are involved in the idiotypic network in myasthenia gravis, and that these functionally different T cells may participate in the immunoregulation of the disease.
重症肌无力患者的外周血T细胞可被人源单克隆抗乙酰胆碱受体抗体和抗独特型抗体刺激分泌γ干扰素和白细胞介素-2。根据细胞因子产生模式，人CD4+ Th细胞可细分为不同亚群。为阐明参与重症肌无力的T细胞亚群，我们分析了两种人疾病特异性单克隆抗体（一种独特型抗体和一种抗独特型抗体）诱导的白细胞介素-4、γ干扰素和白细胞介素-2分泌模式。在34例患者中的33例发现了被刺激分泌一种或多种细胞因子的T细胞。独特型抗体在34例患者中的19例刺激白细胞介素-4分泌，在34例患者中的26例刺激γ干扰素分泌，在34例患者中的25例刺激白细胞介素-2分泌。抗独特型抗体在34例患者中的9例诱导白细胞介素-4分泌，在34例患者中的29例诱导γ干扰素分泌，在34例患者中的28例诱导白细胞介素-2分泌。这些T细胞反应受MHCⅡ类分子限制。根据细胞因子分泌模式，独特型反应性T细胞可能对应于Th1/Th2和/或Th0细胞。抗独特型抗体更频繁地诱导Th1型反应。本研究表明，不同亚群的T淋巴细胞参与重症肌无力的独特型网络，并且这些功能不同的T细胞可能参与该疾病的免疫调节。
